Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences, 37 Chełmońskiego Street, 51-630 Wroclaw, Poland.
Department of Basic Medical Sciences, Faculty of Pharmacy, Wroclaw Medical University, 211 Borowska Street, 50-556 Wrocław, Poland.
Int J Mol Sci. 2022 Aug 19;23(16):9359. doi: 10.3390/ijms23169359.
The goal of this study was to the assess anti-cancer and antioxidant properties of the fruiting body, and to identify bioactive compounds found in their extracts. Significant antiproliferative activity was observed against MCF-7, MCF-7/DX, LOVO, LOVO/DX, MDA-MB 231, SW 620, and NHDF cell lines. With IC values of 25.38 µg/mL and 47.90 µg/mL, respectively, the extract was most effective against MDA-MB 231 and SW 620 cell lines. The bioactive compounds were identified using an ACQUITY UPLC-PDA-MS system. The extracts contained 13 triterpenoids and 28 polyphenols from the flavonols, phenolic acids, flavones, flavan-3-ols, and stilbenes families. Ganoderic acid derivative was found to be the most abundant triterpenoid (162.4 mg/g DW), followed by ganoderic acid B (145.6 mg/g DW). Resveratrol was the most abundant phenolic in the extract (5155.7 mg/100 g DM). The findings could explain why extracts are used in folk medicine.
本研究旨在评估子实体的抗癌和抗氧化特性，并鉴定其提取物中的生物活性化合物。对 MCF-7、MCF-7/DX、LOVO、LOVO/DX、MDA-MB 231、SW 620 和 NHDF 细胞系的增殖表现出显著的抑制作用。提取物对 MDA-MB 231 和 SW 620 细胞系的抑制活性最强，IC 值分别为 25.38 µg/mL 和 47.90 µg/mL。采用 ACQUITY UPLC-PDA-MS 系统对生物活性化合物进行了鉴定。提取物中含有 13 种三萜和 28 种多酚，分别来自于黄酮醇、酚酸、黄酮、黄烷-3-醇和芪类。发现灵芝酸衍生物是最丰富的三萜（162.4 mg/g DW），其次是灵芝酸 B（145.6 mg/g DW）。提取物中含量最丰富的酚类是白藜芦醇（5155.7 mg/100 g DM）。这些发现可以解释为什么提取物在民间医学中被使用。
