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丁香花蕾精油:研磨对化学成分的影响及其与消费者健康安全相关的生物活性

Clove Buds Essential Oil: The Impact of Grinding on the Chemical Composition and Its Biological Activities Involved in Consumer's Health Security.

作者信息

Ben Hassine Dorsaf, Kammoun El Euch Salma, Rahmani Rami, Ghazouani Nessrine, Kane Rouguiata, Abderrabba Manef, Bouajila Jalloul

机构信息

Laboratory Materials, Molecules, and Applications, IPEST, Carthage University, BP 51, La Marsa, Tunisia.

National Agronomic Institute of Tunisia, Food Industries Department, Carthage University, Tunisia.

出版信息

Biomed Res Int. 2021 Aug 2;2021:9940591. doi: 10.1155/2021/9940591. eCollection 2021.

Abstract

This study is aimed at identifying the chemical composition of the essential oil extracted from the seeds, as well as investigating its biological activities, insecticide effect, and allelopathic properties. The extraction yield was about 14.3 and 7.14% for grounded and ungrounded seeds, respectively. The GC-MS analysis allowed the identification of 17 heterogeneous compounds, including eugenol (68.7-87.4%), as major compound, cyperene (20.5-7.2%), phenethyl isovalerate (6.4-3.6%), and -thujopsene (1.9-0.8%), respectively, for grounded and ungrounded seeds. Concerning the antibacterial activity, the diameter of the inhibition zone reached 35 mm when the essential oil extracted from grounded seeds was applied against . Regarding the antioxidant activity the DPPH radical scavenging test, the IC varied from 1.2 ± 0.1 to 2.8 ± 0.5 g/mL. With respect to reducing power, the efficient concentration EC ranged from 32 to 50 g/mL. The essential oil exhibited also an allelopathic effect against seeds of , as well as an insecticide effect against with a DL value of 252.4 L/L air. These findings enhance the use of this spice as a natural food preservative and encourage its use in several fields, including pharmaceutical, cosmetics, agriculture, and therapy, that could be a strategic way to guarantee the consumer's health.

摘要

本研究旨在鉴定从种子中提取的精油的化学成分,同时研究其生物活性、杀虫效果和化感特性。研磨种子和未研磨种子的提取率分别约为14.3%和7.14%。气相色谱 - 质谱联用(GC - MS)分析鉴定出17种不同的化合物,对于研磨种子和未研磨种子,主要化合物分别为丁香酚(68.7 - 87.4%)、石竹烯(20.5 - 7.2%)、异戊酸苯乙酯(6.4 - 3.6%)和α - 崖柏素(1.9 - 0.8%)。关于抗菌活性,当将从研磨种子中提取的精油应用于[具体菌种名称缺失]时,抑菌圈直径达到35毫米。在抗氧化活性方面,通过DPPH自由基清除试验,IC50值在1.2±0.1至2.8±0.5微克/毫升之间变化。关于还原能力,有效浓度EC范围为32至50微克/毫升。该精油对[具体植物名称缺失]的种子也表现出化感作用,并且对[具体昆虫名称缺失]具有杀虫效果,其致死剂量(DL)值为252.4微升/升空气。这些发现增加了这种香料作为天然食品防腐剂的用途,并鼓励其在包括制药、化妆品、农业和治疗等多个领域的应用,这可能是保障消费者健康的一种战略方式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/075e/8352679/8fc3507dd3f6/BMRI2021-9940591.001.jpg

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