Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, People's Republic of China.
Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, People's Republic of China.
Microbiol Res. 2021 Oct;251:126841. doi: 10.1016/j.micres.2021.126841. Epub 2021 Aug 5.
Fusarium equiseti is a pathogenic fungus of plant root rot, and there are few studies on the biocontrol strains of plant wilt caused by F. equiseti. Hence, we conducted a screening and antimicrobial characterization study of marine-origin biocontrol fungi from water samples of the Yap Trench. A new Talaromyces strain DYM25 was screened from water samples of the Yap Trench in the western Pacific Ocean, and its potential as a biocontrol agent against Fusarium wilt of cucumber was studied. 18S rRNA and ITS gene sequencing verified that strain DYM25 belongs to the genus Talaromyces. The growth of F. equiseti was inhibited by strain DYM25 through the antibiosis effect. A preliminary test was first conducted to examine the bioactive stability of filtered DYM25 broth against F. equiseti under various conditions, including high temperature, UV light, alkaline environment, and the presence of metal ions, which indicated its potential as a bio-control agent. The results of the pot experiment showed that F. equiseti caused cucumber wilt, which could be mitigated using the fermentation broth of strain DYM25 (52.9 %). On the other hand, the alkaloid chromogenic reaction showed that the alkaloid salts present in the crude n-butanol extracts were most likely the major components that might have an antimicrobial effect. Therefore, Talaromyces sp. DYM25 represents a new species that can be used as a novel biocontrol agent against cucumber wilt.
禾谷镰刀菌是一种植物根腐病的病原菌，关于其引起的植物枯萎病的生防菌的研究较少。因此，我们对来自西太平洋雅浦海沟水样中的海洋来源的生防真菌进行了筛选和抗菌特性研究。从西太平洋雅浦海沟水样中筛选到一株新的拟青霉菌株 DYM25，研究了其对黄瓜枯萎病的生防潜力。18S rRNA 和 ITS 基因测序证实菌株 DYM25 属于拟青霉属。通过抑菌作用抑制禾谷镰刀菌的生长。首先对 DYM25 过滤培养液在不同条件下（高温、紫外线、碱性环境和金属离子存在）对禾谷镰刀菌的生物活性稳定性进行了初步测试，结果表明其具有生防剂的潜力。盆栽试验结果表明，禾谷镰刀菌引起黄瓜枯萎病，用菌株 DYM25 的发酵液（52.9%）可减轻枯萎病。另一方面，生物碱显色反应表明，粗正丁醇提取物中的生物碱盐很可能是具有抗菌作用的主要成分。因此，拟青霉 DYM25 代表了一种可用于防治黄瓜枯萎病的新型生防菌。
