State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, People's Republic of China.
Molecular Imaging Program at Stanford (MIPS), Bio-X Program and Department of Radiology, Canary Center at Stanford for Cancer Early Detection, Stanford University, Stanford, CA, USA.
Nat Biomed Eng. 2022 May;6(5):629-639. doi: 10.1038/s41551-021-00773-2. Epub 2021 Aug 12.
In the second near-infrared spectral window (NIR-II; with wavelengths of 1,000-1,700 nm), in vivo fluorescence imaging can take advantage of reduced tissue autofluorescence and lower light absorption and scattering by tissue. Here, we report the development and in vivo application of a NIR-II phosphorescent probe that has lifetimes of hundreds of microseconds and a Stokes shift of 430 nm. The probe is made of glutathione-capped copper-indium-selenium nanotubes, and in acidic environments (pH 5.5-6.5) switches from displaying fluorescence to phosphorescence. In xenograft models of osteosarcoma and breast cancer, intravenous or intratumoral injections of the probe enabled phosphorescence imaging at signal-to-background ratios, spatial resolutions and sensitivities higher than NIR-II fluorescence imaging with polymer-stabilized copper-indium-sulfide nanorods. Phosphorescence imaging may offer superior imaging performance for a range of biomedical uses.
在第二个近红外光谱窗口（NIR-II；波长为 1000-1700nm）中，体内荧光成像是利用减少的组织自发荧光和更低的组织光吸收和散射来实现的。在这里，我们报告了一种 NIR-II 磷光探针的开发和体内应用，该探针具有数百微秒的寿命和 430nm 的斯托克斯位移。该探针由谷胱甘肽封端的铜-铟-硒纳米管组成，在酸性环境（pH 5.5-6.5）下，从显示荧光转变为磷光。在骨肉瘤和乳腺癌的异种移植模型中，静脉内或肿瘤内注射探针，使磷光成像的信号与背景比、空间分辨率和灵敏度都高于聚合物稳定的铜-铟-硫化纳米棒的 NIR-II 荧光成像。磷光成像是一系列生物医学应用中具有优越成像性能的方法。
