Singleton David A, Pongchaikul Pisut, Smith Shirley, Bengtsson Rebecca J, Baker Kate, Timofte Dorina, Steen Stephen, Jones Matthew, Roberts Larry, Sánchez-Vizcaíno Fernando, Dawson Susan, Noble P-J M, Radford Alan D, Pinchbeck Gina L, Williams Nicola J
Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Neston, United Kingdom.
Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Salaya, Thailand.
Front Microbiol. 2021 Jul 30;12:700698. doi: 10.3389/fmicb.2021.700698. eCollection 2021.
Antimicrobial resistance (AMR) is a globally important one health threat. The impact of resistant infections on companion animals, and the potential public health implications of such infections, has not been widely explored, largely due to an absence of structured population-level data.
We aimed to efficiently capture and repurpose antimicrobial susceptibility test (AST) results data from several veterinary diagnostic laboratories (VDLs) across the United Kingdom to facilitate national companion animal clinical AMR surveillance. We also sought to harness and genotypically characterize isolates of potential AMR importance from these laboratories.
We summarized AST results for 29,330 canine and 8,279 feline isolates originating from companion animal clinical practice, performed between April 2016 and July 2018 from four VDLs, with submissions from 2,237 United Kingdom veterinary practice sites.
() was the most commonly isolated in dogs (69.4% of AST results, 95% confidence interval, CI, 68.7-70.0) and cats (90.5%, CI 89.8-91.3). Multi-drug resistance was reported in 14.1% (CI 13.5-14.8) of canine and 12.0% (CI 11.1-12.9) of feline isolates. Referral practices were associated with increased 3rd generation ≤ cephalosporin resistance odds (dogs: odds ratio 2.0, CI 1.2-3.4). We selected 95 isolates for whole genome analyses, of which seven belonged to sequence type 131, also carrying the plasmid-associated extended spectrum β-lactamase gene . The plasmid-mediated colistin resistance gene was also identified for the first time in companion animals.
Linking clinical AMR data with genotypic characterization represents an efficient means of identifying important resistance trends in companion animals on a national scale.
抗菌药物耐药性（AMR）是对全球“同一健康”构成重要威胁的问题。耐药性感染对伴侣动物的影响以及此类感染对公共卫生的潜在影响尚未得到广泛研究，这主要是由于缺乏结构化的群体层面数据。
我们旨在有效获取并重新利用来自英国多个兽医诊断实验室（VDL）的抗菌药物敏感性试验（AST）结果数据，以促进全国伴侣动物临床AMR监测。我们还试图利用这些实验室中具有潜在AMR重要性的分离株并对其进行基因分型特征分析。
我们总结了2016年4月至2018年7月期间来自四个VDL、由2237个英国兽医诊疗机构提交的、源自伴侣动物临床实践的29330份犬类和8279份猫类分离株的AST结果。
（此处原文缺失具体内容）是犬类（占AST结果的69.4%，95%置信区间，CI，68.7 - 70.0）和猫类（90.5%，CI 89.8 - 91.3）中最常分离出的（此处原文缺失具体内容）。在14.1%（CI 13.5 - 14.8）的犬类分离株和12.0%（CI 11.1 - 12.9）的猫类分离株中报告了多重耐药性。转诊诊疗机构与第三代≤头孢菌素耐药几率增加相关（犬类：优势比为2.0，CI 1.2 - 3.4）。我们选择了95份分离株进行全基因组分析，其中7份属于序列类型131，也携带与质粒相关的超广谱β - 内酰胺酶基因（此处原文缺失具体内容）。质粒介导的黏菌素耐药基因也首次在伴侣动物中被鉴定出来。
将临床AMR数据与基因分型特征分析相结合是在全国范围内识别伴侣动物重要耐药趋势的有效手段。
