Blažević Ivica, Đulović Azra, Maravić Ana, Čikeš Čulić Vedrana, Montaut Sabine, Rollin Patrick
Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000, Split, Croatia.
Department of Biology, Faculty of Science, University of Split, Ruđera Boškovića 33, 21000, Split, Croatia.
Chem Biodivers. 2019 Apr;16(4):e1800661. doi: 10.1002/cbdv.201800661. Epub 2019 Mar 20.
The cultivated Lepidium latifolium L. was investigated to decipher its glucosinolate profile, antimicrobial, and cytotoxic activities. HPLC/ESI-MS analyses of the intact glucosinolates and GC/MS analysis of their hydrolysis products showed the presence of sinigrin (1), glucocochlearin (2), glucotropaeolin (3), and 4-methoxyglucobrassicin (4). Hydrodistillate, extract, and allyl isothiocyanate, the main volatile resulting from sinigrin degradation, showed antimicrobial activity against all eleven tested pathogenic and food spoilage bacteria and fungi, with highest effect observed against Candida albicans with MIC 8 and 16 μg/mL. Hydrodistillate and extract showed the best cytotoxic activity on bladder cancer UM-UC-3 cell line during an incubation time of 24 h (IC 192.9 and 133.8 μg/mL, respectively), while the best effect on glioblastoma LN229 cell line was observed after 48 h (IC 110.8 and 30.9 μg/mL, respectively). Pure allyl isothiocyanate displayed a similar trend in cytotoxic effect on both cell lines (IC 23.3 and 36.5 μg/mL after 24 h and 48 h, respectively).
对栽培的宽叶独行菜进行了研究，以解析其硫代葡萄糖苷谱、抗菌和细胞毒性活性。完整硫代葡萄糖苷的HPLC/ESI-MS分析及其水解产物的GC/MS分析表明存在黑芥子硫苷（1）、葡糖耳蜗苷（2）、葡糖异硫氰酸苯乙酯（3）和4-甲氧基葡糖芸苔素（4）。水蒸馏液、提取物以及黑芥子硫苷降解产生的主要挥发性成分烯丙基异硫氰酸酯对所有11种受试致病细菌、食品腐败细菌和真菌均显示出抗菌活性，对白色念珠菌的抗菌效果最佳，MIC为8和16μg/mL。在24小时的孵育时间内，水蒸馏液和提取物对膀胱癌UM-UC-3细胞系显示出最佳的细胞毒性活性（IC分别为192.9和133.8μg/mL），而在48小时后对胶质母细胞瘤LN229细胞系观察到最佳效果（IC分别为110.8和30.9μg/mL）。纯烯丙基异硫氰酸酯对两种细胞系的细胞毒性作用呈现相似趋势（24小时和48小时后的IC分别为23.3和36.5μg/mL）。
