Cancer Biology Research Center (Key Laboratory of the Ministry of Education), Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Microb Ecol. 2022 Jul;84(1):302-313. doi: 10.1007/s00248-021-01800-0. Epub 2021 Aug 17.
The vaginal microbiota is closely related to HPV infection and cervical cancer (CC), but its relationship with platinum-based chemotherapy responsiveness is unknown. The study aimed to investigate the vaginal microbiota diversity of women with locally advanced cervical cancer (LACC) and compare the differences between responders and nonresponders. We characterized the 16S rRNA gene sequencing of vaginal microbiome of 66 vaginal samples, including 26 LACC patients before neoadjuvant chemotherapy and 40 healthy controls. Compared with the healthy controls, alpha diversity was significantly increased in CC patients (p <0.05) with more unconventionality bacterial colonization. Beta diversity also significantly differed between cervical cancer patients and controls (p <0.01). Within the CC patients, alpha diversity in vaginal samples was significantly higher in the nonresponders versus the responders (p <0.01), and the Ace index and Chao index were negatively correlated with mass reduction (p <0.001). Moreover, the Bacteroides genus enriched in the nonresponders had a ROC-plot AUC value reaching 0.84. The study suggests the vaginal microbiota in LACC patients is associated with platinum-based chemotherapy responsiveness. Alpha diversity and Bacteroides abundance have the potential of identifying platinum-resistant patients at an early time. These findings provide a basis for further research on the relationship between vaginal microbiome and chemotherapy effect in LACC.
阴道微生物群与 HPV 感染和宫颈癌（CC）密切相关，但与铂类化疗反应性的关系尚不清楚。本研究旨在调查局部晚期宫颈癌（LACC）女性的阴道微生物群多样性，并比较反应者和非反应者之间的差异。我们对 66 个阴道样本的 16S rRNA 基因测序进行了特征分析，其中包括 26 名接受新辅助化疗前的 LACC 患者和 40 名健康对照。与健康对照组相比，CC 患者的 alpha 多样性显着增加（p<0.05），非常规细菌定植更多。beta 多样性也在宫颈癌患者和对照组之间存在显着差异（p<0.01）。在 CC 患者中，非反应者的阴道样本 alpha 多样性显着高于反应者（p<0.01），Ace 指数和 Chao 指数与质量减少呈负相关（p<0.001）。此外，非反应者中富集的拟杆菌属具有达到 0.84 的 ROC 曲线 AUC 值。本研究表明，LACC 患者的阴道微生物群与铂类化疗反应性有关。alpha 多样性和拟杆菌属丰度有可能在早期识别出铂类耐药患者。这些发现为进一步研究 LACC 中阴道微生物群与化疗效果之间的关系提供了基础。
