患者产生的 SARS-CoV-2 气溶胶的大小和可培养性。
The size and culturability of patient-generated SARS-CoV-2 aerosol.
机构信息
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.
Global Center for Health Security, University of Nebraska Medical Center, Omaha, NE, USA.
出版信息
J Expo Sci Environ Epidemiol. 2022 Sep;32(5):706-711. doi: 10.1038/s41370-021-00376-8. Epub 2021 Aug 18.
BACKGROUND
Aerosol transmission of COVID-19 is the subject of ongoing policy debate. Characterizing aerosol produced by people with COVID-19 is critical to understanding the role of aerosols in transmission.
OBJECTIVE
We investigated the presence of virus in size-fractioned aerosols from six COVID-19 patients admitted into mixed acuity wards in April of 2020.
METHODS
Size-fractionated aerosol samples and aerosol size distributions were collected from COVID-19 positive patients. Aerosol samples were analyzed for viral RNA, positive samples were cultured in Vero E6 cells. Serial RT-PCR of cells indicated samples where viral replication was likely occurring. Viral presence was also investigated by western blot and transmission electron microscopy (TEM).
RESULTS
SARS-CoV-2 RNA was detected by rRT-PCR in all samples. Three samples confidently indicated the presence of viral replication, all of which were from collected sub-micron aerosol. Western blot indicated the presence of viral proteins in all but one of these samples, and intact virions were observed by TEM in one sample.
SIGNIFICANCE
Observations of viral replication in the culture of submicron aerosol samples provides additional evidence that airborne transmission of COVID-19 is possible. These results support the use of efficient respiratory protection in both healthcare and by the public to limit transmission.
背景
COVID-19 的气溶胶传播是当前政策辩论的主题。描述 COVID-19 患者产生的气溶胶对于了解气溶胶在传播中的作用至关重要。
目的
我们调查了 2020 年 4 月六名入住混合 acuity 病房的 COVID-19 患者的大小分级气溶胶中是否存在病毒。
方法
从 COVID-19 阳性患者中采集大小分级的气溶胶样本和气溶胶粒径分布。对气溶胶样本进行病毒 RNA 分析，对阳性样本在 Vero E6 细胞中进行培养。细胞的连续 RT-PCR 表明可能发生了病毒复制。还通过 Western blot 和透射电子显微镜（TEM）研究了病毒的存在。
结果
rRT-PCR 在所有样本中均检测到 SARS-CoV-2 RNA。三个样本明确表明存在病毒复制，均来自采集的亚微米气溶胶。Western blot 表明除一个样本外，所有这些样本中均存在病毒蛋白，一个样本中通过 TEM 观察到完整的病毒颗粒。
意义
在亚微米气溶胶样本的培养中观察到病毒复制，为 COVID-19 的空气传播可能性提供了更多证据。这些结果支持在医疗保健和公众中使用高效的呼吸保护措施来限制传播。
Zotero 插件