Kottenhahn Patrick, Philipps Gabriele, Jennewein Stefan
Department for Industrial Biotechnology, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstr. 6, 52074, Aachen, Germany.
Heliyon. 2021 Aug 5;7(8):e07732. doi: 10.1016/j.heliyon.2021.e07732. eCollection 2021 Aug.
converts syngas into industrial alcohols like hexanol, but titers may be limited by product toxicity. Investigation of IC at 30 °C (17.5 mM) and 37 °C (11.8 mM) revealed increased hexanol tolerance at lower temperatures. To avoid product toxicity, oleyl alcohol was added as an extraction solvent, increasing hexanol production nearly 2.5-fold to 23.9 mM (2.4 g/L) at 30 °C. This titer exceeds the concentration that is acutely toxic in the absence of a solvent, confirming the hypothesis that current hexanol production is limited by product toxicity. The solvent however had no positive effect at 37 °C. Furthermore, cell membranes adapted to the higher temperature by incorporating more saturated fatty acids, but surprisingly not to hexanol. Corn oil and sunflower seed oil were tested as alternative, inexpensive extraction solvents. Hexanol titers were similar with all solvents, but oleyl alcohol achieved the highest extraction efficiency.
将合成气转化为工业醇类，如己醇，但产量可能受产物毒性限制。在30°C（17.5 mM）和37°C（11.8 mM）下对己醇耐受性的研究表明，较低温度下己醇耐受性增强。为避免产物毒性，添加油醇作为萃取溶剂，在30°C时己醇产量增加近2.5倍，达到23.9 mM（2.4 g/L）。该产量超过了在无溶剂时具有急性毒性的浓度，证实了当前己醇生产受产物毒性限制的假设。然而，该溶剂在37°C时没有积极作用。此外，细胞膜通过掺入更多饱和脂肪酸来适应较高温度，但令人惊讶的是对己醇不适应。玉米油和葵花籽油作为替代的廉价萃取溶剂进行了测试。所有溶剂的己醇产量相似，但油醇的萃取效率最高。
