Deutsch Alana, McLellan Beth N, Shinoda Kosaku
Division of Dermatology, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York.
Division of Endocrinology and Diabetes, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York.
JAAD Int. 2020 Oct 6;1(2):182-188. doi: 10.1016/j.jdin.2020.08.001. eCollection 2020 Dec.
The skin is an ecosystem composed of specialized cell types that work together to serve as a physical protective barrier. Single-cell resolution is therefore essential to deconvolve skin's heterogeneity by identifying novel, distinct cell subsets in health and disease. Single-cell RNA sequencing is a highly meticulous methodology used to study the distinct transcriptional profiles of each cell within large tissue libraries at uniquely high resolution. The investigative capabilities achieved by this methodology allow previously unattainable analyses, including identification of rare cell populations, evaluation of cell-to-cell variation, and the ability to track trajectories of distinct cell lineages through development. In the past decade, application of transcriptomic analysis to skin biology and dermatology has greatly advanced understanding of homeostatic physiology in the skin, as well as a multitude of dermatologic diseases. Single-cell RNA sequencing offers tremendous promise for identification of novel therapeutic targets in dermatologic diseases, with broad implications of improving therapeutic interventions.
皮肤是一个由特殊细胞类型组成的生态系统，这些细胞协同工作以形成物理保护屏障。因此，单细胞分辨率对于通过识别健康和疾病状态下新的、不同的细胞亚群来解析皮肤的异质性至关重要。单细胞RNA测序是一种高度精细的方法，用于以独特的高分辨率研究大型组织文库中每个细胞的独特转录谱。这种方法所实现的研究能力使得以前无法进行的分析成为可能，包括识别稀有细胞群体、评估细胞间变异以及追踪不同细胞谱系在发育过程中的轨迹。在过去十年中，转录组分析在皮肤生物学和皮肤病学中的应用极大地推进了我们对皮肤稳态生理学以及多种皮肤病的理解。单细胞RNA测序为识别皮肤病中的新治疗靶点带来了巨大希望，对改善治疗干预具有广泛意义。
