Pathogenicity and Metabolites of Endoparasitic Nematophagous Fungus YMF1.01759 against Nematodes.

Plant parasitic nematodes cause severe damage to crops. Endoparasitic nematophagous fungi (ENF) are a type of important biocontrol fungi, which can cause disease or kill nematodes by producing various spores. As a major ENF, displays certain potential for controlling plant-parasitic nematodes. In this study, the pathogenicity and secondary metabolites of the endoparasitic fungus YMF1.01759 were investigated. The strain YMF1.01759 had high infection efficiency against nematodes. The process of infecting nematodes by the strain was observed under an electron microscope. Here, 13 metabolites including one new compound 4()-butoxy-3-(butoxymethyl)-2-hydroxycyclopent-2-en-1-one () were isolated and identified from the fermentation products of YMF1.01759 cultured in a SDAY solid medium. Furthermore, a bioassay showed that 5-hydroxymethylfuran-2-carboxylic acid () is toxic to the root knot nematode and affects the hatching of its egg. Thereby, the nematicidal mortality attained 81.50% at 100 μg/mL for 48 h. Furthermore, egg hatching was inhibited at the tested concentrations, compared with water control eggs. This is the first report on the secondary metabolites of the ENF . The results indicated that could infect nematodes by spores and produce active metabolites to kill nematodes. The biological control potential of against nematodes was expounded further.