Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands (UIB), Ctra. Valldemossa km 7.5, 07122 Palma, Spain.
Health Research Institute of the Balearic Islands (IdISBa), 07120 Palma, Spain.
Int J Mol Sci. 2021 Aug 10;22(16):8580. doi: 10.3390/ijms22168580.
Extracellular vesicles (EVs) present a great potential for the development of new treatments in the biomedical field. To be used as therapeutics, many different sources have been used for EVs obtention, while only a few studies have addressed the use of platelet-derived EVs (pEVs). In fact, pEVs have been shown to intervene in different healing responses, thus some studies have evaluated their regenerative capability in wound healing or hemorrhagic shock. Even more, pEVs have proven to induce cellular differentiation, enhancing musculoskeletal or neural regeneration. However, the obtention and characterization of pEVs is widely heterogeneous and differs from the recommendations of the International Society for Extracellular Vesicles. Therefore, in this review, we aim to present the main advances in the therapeutical use of pEVs in the regenerative medicine field while highlighting the isolation and characterization steps followed. The main goal of this review is to portray the studies performed in order to enhance the translation of the pEVs research into feasible therapeutical applications.
细胞外囊泡 (EVs) 在生物医学领域的新疗法开发中具有巨大潜力。为了用作治疗方法，已经使用了许多不同的来源来获取 EVs，而只有少数研究涉及血小板衍生的 EVs (pEVs) 的使用。事实上，pEVs 已被证明可以干预不同的愈合反应，因此一些研究评估了它们在伤口愈合或失血性休克中的再生能力。更重要的是，pEVs 已被证明能诱导细胞分化，增强肌肉骨骼或神经再生。然而，pEVs 的获得和表征广泛存在异质性，与细胞外囊泡国际协会的建议不同。因此，在这篇综述中，我们旨在介绍 pEVs 在再生医学领域的治疗用途的主要进展，同时强调所遵循的分离和表征步骤。本综述的主要目的是描述为了将 pEVs 的研究转化为可行的治疗应用而进行的研究。
