Oh Eun-Seok, Park Hyeonseon, Lee Kwanuk, Shim Donghwan, Oh Man-Ho
Department of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea.
Department of Biology, College of Natural Sciences, Jeju National University, Jeju 63243, Republic of Korea.
Plants (Basel). 2024 Aug 5;13(15):2167. doi: 10.3390/plants13152167.
Clubroot, caused by , is one of the diseases that causes major economic losses in cruciferous crops worldwide. Although prevention strategies, including soil pH adjustment and crop rotation, have been used, the disease's long persistence and devastating impact continuously remain in the soil. CR varieties were developed for clubroot-resistant (CR) Chinese cabbage, and 'Akimeki' is one of the clubroot disease-resistant cultivars. However, recent studies have reported susceptibility to several Korean pathotypes in Akimeki and the destruction of the resistance to in many Brassica species against CR varieties, requiring the understanding of more fine-tuned plant signaling by fungal pathogens. In this study, we focused on the early molecular responses of Akimeki during infection with two strains, Seosan (SS) and Hoengseong2 (HS2), using RNA sequencing (RNA-seq). Among a total of 2358 DEGs, 2037 DEGs were differentially expressed following SS and HS2 infection. Gene ontology (GO) showed that 1524 and 513 genes were up-regulated following SS and HS2 inoculations, respectively. Notably, the genes of defense response and jasmonic acid regulations were enriched in the SS inoculation condition, and the genes of water transport and light intensity response were enriched in the HS2 inoculation condition. Moreover, KEGG pathways revealed that the gene expression set were related to pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) mechanisms. The results will provide valuable information for developing CR cultivars in Brassica plants.
根肿病是由[病原体名称缺失]引起的，是导致全球十字花科作物重大经济损失的病害之一。尽管已经采用了包括土壤pH调节和作物轮作在内的预防策略，但这种病害在土壤中的长期存在和毁灭性影响依然持续。已培育出抗根肿病（CR）的大白菜品种，“秋光”就是其中一种抗根肿病的栽培品种。然而，最近的研究报告称，“秋光”对几种韩国致病型敏感，并且许多芸苔属物种对CR品种的抗性遭到破坏，这就需要了解真菌病原体对植物信号更精细的调控。在本研究中，我们利用RNA测序（RNA-seq）技术，重点研究了“秋光”在感染两种[病原体名称缺失]菌株（瑞山（SS）和横城2（HS2））过程中的早期分子反应。在总共2358个差异表达基因（DEG）中，有2037个DEG在感染SS和HS2后差异表达。基因本体论（GO）显示，分别有1524个和513个基因在接种SS和HS2后上调。值得注意的是，防御反应和茉莉酸调控相关基因在接种SS的条件下富集，而水分运输和光强反应相关基因在接种HS2的条件下富集。此外，京都基因与基因组百科全书（KEGG）通路显示，基因表达集与模式触发免疫（PTI）和效应子触发免疫（ETI）机制有关。这些结果将为芸苔属植物CR品种的培育提供有价值的信息。
