Bere Antonia Windkouni, Mulati Omuyundo, Kimotho James, Ng'ong'a Florence
Department of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation, Nairobi P.O. Box 62000-00200, Kenya.
Department of Biomedical Sciences and Technology, Technical University of Kenya, Nairobi P.O. Box 52428-00200, Kenya.
Pharmaceuticals (Basel). 2021 Jul 26;14(8):718. doi: 10.3390/ph14080718.
The current global occurrence of dengue infection annually is approximately 400 million, with a case fatality rate of 2.5%. However, there are no antiviral agents. leaf extract is known for its medicinal value, due to the presence of organic compounds that possess antimicrobial, anti-inflammatory, and antioxidant activities. This study determined the anti-dengue effect of leaf extract silver synthesized nanoparticles. In this study, aqueous and non-aqueous extractions were carried out, followed by the synthesis of silver nanoparticles as well as characterization through Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy. The in vitro anti-dengue effect was evaluated using a focus reduction neutralization test on kidney Vero E2 cell lines. In silico studies involved molecular docking to determine the potential interactions between the bioactive compounds in leaf extract and the viral NS5 protein. leaf methanol extract silver synthesized nanoparticle was the most promising with an IC of 9.20 µg/mL. Molecular docking showed 5,7 dimethoxycoumarin as the best ligand, with binding energy of -7.75 kcal/mol, indicating high affinity for the NS5 protein. These results highlight that leaf methanol extract silver synthesized nanoparticles could be used to inhibit dengue virus type 2 viral replication. However, we recommend further studies to determine their toxicity and the safety profiles.
目前全球每年登革热感染病例约为4亿，病死率为2.5%。然而，尚无抗病毒药物。叶提取物因其含有具有抗菌、抗炎和抗氧化活性的有机化合物而具有药用价值。本研究确定了叶提取物银合成纳米颗粒的抗登革热作用。在本研究中，进行了水相和非水相提取，随后合成了银纳米颗粒，并通过傅里叶变换红外光谱（FTIR）和扫描电子显微镜进行了表征。使用焦点减少中和试验在肾Vero E2细胞系上评估体外抗登革热作用。计算机模拟研究涉及分子对接，以确定叶提取物中的生物活性化合物与病毒NS5蛋白之间的潜在相互作用。叶甲醇提取物银合成纳米颗粒最具前景，IC为9.20 µg/mL。分子对接显示5,7-二甲氧基香豆素为最佳配体，结合能为-7.75 kcal/mol，表明对NS5蛋白具有高亲和力。这些结果突出表明，叶甲醇提取物银合成纳米颗粒可用于抑制2型登革热病毒的复制。然而，我们建议进一步研究以确定其毒性和安全性。
