和 的绿色合成银纳米粒子的抗病毒潜力。
Antiviral potential of green synthesized silver nanoparticles of and .
机构信息
Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
出版信息
Int J Nanomedicine. 2019 Aug 6;14:6217-6229. doi: 10.2147/IJN.S214171. eCollection 2019.
BACKGROUND
Viral and microbial infections constitute one of the most important life-threatening problems. The emergence of new viral and bacterial infectious diseases increases the demand for new therapeutic drugs.
PURPOSE
The objective of this study was to use the aqueous and hexane extracts of and F. Aizoaceae for the synthesis of silver nanoparticles, and to investigate its possible antiviral activity. In addition to the investigation of the phytochemical composition of the crude methanolic extracts of the two plants through UPLC-MS metabolomic profiling, and it was followed by molecular docking in order to explore the chemical compounds that might contribute to the antiviral potential.
METHODS
The formation of SNPs was further confirmed using a transmission electron microscope (TEM), UV-Visible spectroscopy and Fourier transform infrared spectroscopy. The antiviral activity of the synthesized nanoparticles was evaluated using MTT assay against HSV-1, HAV-10 virus and Coxsackie B4 virus. Metabolomics profiling was performed using UPLC-MS and molecular docking was performed via Autodock4 and visualization was done using the Discovery studio.
RESULTS
The early signs of SNPs synthesis were detected by a color change from yellow to reddish brown color. The TEM analysis of SNPs showed spherical nanoparticles with mean size ranges between 10.12 nm to 27.89 nm, and 8.91 nm 14.48 nm for and aqueous and hexane extracts respectively. The UV-Visible spectrophotometric analysis showed an absorption peak at λmax of 417 nm.The green synthesized SNPs of and showed remarkable antiviral activity against HSV-1, HAV-10, and CoxB4 virus. Metabolomics profiling of the methanolic extract of and resulted in identifying 12 compounds. The docking study predicted the patterns of interactions between the compounds of and with herpes simplex thymidine kinase, hepatitis A 3c proteinase, and Coxsackievirus B4 3c protease, which was similar to those of the co-crystal inhibitors and this can provide a supposed explanation for the antiviral activity of the aqueous and nano extracts of and .
CONCLUSION
These results highlight that SNPs of and could have antiviral activity against HSV-1, HAV-10, and CoxB4 virus.
背景
病毒和微生物感染是最具威胁生命的问题之一。新的病毒性和细菌性传染病的出现增加了对新治疗药物的需求。
目的
本研究旨在使用 和 F. Aizoaceae 的水提物和正己烷提取物合成银纳米粒子，并研究其可能的抗病毒活性。除了通过 UPLC-MS 代谢组学分析研究两种植物粗甲醇提取物的植物化学成分外，还进行了分子对接，以探索可能有助于抗病毒潜力的化学化合物。
方法
通过透射电子显微镜 (TEM)、紫外可见光谱和傅里叶变换红外光谱进一步证实 SNP 的形成。使用 MTT 测定法评估合成纳米粒子对 HSV-1、HAV-10 病毒和柯萨奇 B4 病毒的抗病毒活性。使用 UPLC-MS 进行代谢组学分析，通过 Autodock4 进行分子对接，使用 Discovery studio 进行可视化。
结果
通过颜色从黄色变为红棕色，检测到 SNP 合成的早期迹象。SNP 的 TEM 分析显示，球形纳米粒子的平均粒径范围分别为 10.12nm 至 27.89nm 和 8.91nm 至 14.48nm， 和 水提物和正己烷提取物分别为 10.12nm 至 27.89nm 和 8.91nm 至 14.48nm。紫外可见分光光度分析显示在 λmax 处有吸收峰 417nm。 和 合成的绿色纳米粒子对 HSV-1、HAV-10 和 CoxB4 病毒表现出显著的抗病毒活性。 和 甲醇提取物的代谢组学分析导致鉴定出 12 种化合物。对接研究预测了 和 化合物与单纯疱疹胸苷激酶、甲型肝炎 3c 蛋白酶和柯萨奇病毒 B4 3c 蛋白酶之间相互作用的模式，与共晶抑制剂的模式相似，这可以为 和 水提物和纳米提取物的抗病毒活性提供假设性解释。
结论
这些结果表明， 和 合成的 SNP 可能对 HSV-1、HAV-10 和 CoxB4 病毒具有抗病毒活性。
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