新型纳米氟化钙再矿化抗菌牙科复合材料。

Novel Nano Calcium Fluoride Remineralizing and Antibacterial Dental Composites.

机构信息

Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, United States; Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh 11451, Saudi Arabia.

Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, United States; Department of Restorative Dental Sciences, College of Dentistry, Umm Al-Qura University, Makkah 24211, Saudi Arabia.

出版信息

J Dent. 2021 Oct;113:103789. doi: 10.1016/j.jdent.2021.103789. Epub 2021 Aug 26.

DOI:10.1016/j.jdent.2021.103789

PMID:34455017

Abstract

OBJECTIVE

Composites with remineralizing and antibacterial properties are favorable for caries inhibition. The objectives of this study were to develop a new bioactive nanocomposite with remineralizing and antibiofilm properties by incorporating dimethylaminohexadecyl methacrylate (DMAHDM) and nano-calcium fluoride (nCaF).

METHODS

nCaF was produced via a spray-drying method and integrated at 15% mass fraction into composite. DMAHDM was added at 3% mass fraction. Mechanical properties and F and Ca ion releases were assessed. Colony-forming units (CFU), lactic acid and metabolic activity of biofilms on composites were performed.

RESULTS

The new composites had flexural strengths of (95.28±6.32) MPa and (125.93±7.49) MPa, which were within the ISO recommendations. Biofilm CFU were reduced by 3-4 log (p<0.05). The composites achieved high F releases of (0.89±0.01) mmol/L and (0.44±0.01) mmol/L, and Ca releases of (1.46±0.05) mmol/L and (0.54±0.005) mmol/L.

CONCLUSIONS

New nanocomposites were developed with good mechanical properties, potent antibacterial activity against salivary biofilms, and high F and Ca ion releases with potential for remineralization.

CLINICAL SIGNIFICANCE

Novel nanocomposites using nCaF and DMAHDM were developed with potent antibacterial and remineralizing effects and high F and Ca ion releases. They are promising to inhibit recurrent caries, promote remineralization, and possess long-term sustainability.

摘要

目的

具有再矿化和抗菌性能的复合材料有利于抑制龋齿。本研究的目的是通过掺入二甲基氨基己基甲基丙烯酸酯（DMAHDM）和纳米氟化钙（nCaF）来开发具有再矿化和抗生物膜特性的新型生物活性纳米复合材料。

方法

通过喷雾干燥法制备 nCaF，并以 15%的质量分数掺入复合材料中。添加 3%质量分数的 DMAHDM。评估了机械性能以及 F 和 Ca 离子的释放情况。对复合材料上的菌落形成单位（CFU）、乳酸和生物膜代谢活性进行了检测。

结果

新型复合材料的抗弯强度为（95.28±6.32）MPa 和（125.93±7.49）MPa，在 ISO 建议范围内。生物膜 CFU 减少了 3-4 对数（p<0.05）。复合材料的 F 释放量高达（0.89±0.01）mmol/L 和（0.44±0.01）mmol/L，Ca 释放量高达（1.46±0.05）mmol/L 和（0.54±0.005）mmol/L。