Perrin Simon, Julien Anais, de Lageneste Oriane Duchamp, Abou-Khalil Rana, Colnot Céline
Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France.
Center of Excellence for Biomedicine, Joint Centers of Excellence Program, King Abdulaziz City for Science and Technology (KACST), Riyadh, Saudi Arabia.
Bio Protoc. 2021 Aug 5;11(15):e4107. doi: 10.21769/BioProtoc.4107.
The periosteum covering the outer surface of bone contains skeletal stem/progenitor cells that can efficiently form cartilage and bone during bone repair. Several methods have been described to isolate periosteal cells based on bone scraping and/or enzymatic digestion. Here, we describe an explant culture method to isolate periosteum-derived stem/progenitor cells for subsequent and analyses. Periosteal cells (PCs) isolated using this protocol express mesenchymal markers, can be expanded , and exhibit high regenerative potential after transplantation at a fracture site, suggesting that this protocol can be employed for PC production to use in new cell-based therapies.
覆盖在骨外表面的骨膜含有骨骼干/祖细胞，这些细胞在骨修复过程中能够有效地形成软骨和骨。已经描述了几种基于骨刮除和/或酶消化来分离骨膜细胞的方法。在此，我们描述一种外植体培养方法，用于分离骨膜来源的干/祖细胞以便后续研究和分析。使用该方案分离的骨膜细胞（PCs）表达间充质标志物，能够扩增，并且在骨折部位移植后表现出高再生潜力，这表明该方案可用于生产PCs以用于新的基于细胞的治疗。
