Institute of Ophthalmology, University College London, London, United Kingdom.
PLoS One. 2021 Sep 3;16(9):e0257149. doi: 10.1371/journal.pone.0257149. eCollection 2021.
Increased blue light exposure has become a matter of concern as it has a range of detrimental effects, but the mechanisms remain unclear. Mitochondria absorb short wavelength light but have a specific absorbance at 420nm at the lower end of the human visual range. This 420nm absorption is probably due to the presence of porphyrin. We examine the impact of 420nm exposure on drosophila melanogaster mitochondria and its impact on fly mobility. Daily 15 mins exposures for a week significantly reduced mitochondrial complex activities and increased mitochondrial inner membrane permeability, which is a key metric of mitochondrial health. Adenosine triphosphate (ATP) levels were not significantly reduced and mobility was unchanged. There are multiple options for energy/time exposure combinations, but we then applied single 420nm exposure of 3h to increase the probability of an effect on ATP and mobility, and both were significantly reduced. ATP and mitochondrial membrane permeability recovered and over corrected at 72h post exposure. However, despite this, normal mobility did not return. Hence, the effect of short wavelengths on mitochondrial function is to reduce complex activity and increasing membrane permeability, but light exposure to reduce ATP and to translate into reduced mobility needs to be sustained.
随着蓝光照射量的增加，人们越来越关注其带来的一系列有害影响，但其中的作用机制仍不清楚。线粒体吸收短波长的光，但在人类视觉范围的低端，在 420nm 处有一个特定的吸收峰。这种 420nm 的吸收可能是由于卟啉的存在。我们研究了 420nm 照射对黑腹果蝇线粒体的影响及其对果蝇运动能力的影响。一周内每天 15 分钟的暴露显著降低了线粒体复合物的活性，并增加了线粒体内膜的通透性，这是线粒体健康的一个关键指标。三磷酸腺苷（ATP）水平没有明显降低，运动能力也没有改变。有多种能量/时间暴露组合可供选择，但我们随后应用了 3 小时的单次 420nm 暴露，以增加对 ATP 和运动能力的影响的可能性，这两者都显著降低。暴露后 72 小时，ATP 和线粒体膜通透性恢复并过度校正。然而，尽管如此，正常的运动能力并没有恢复。因此，短波长对线粒体功能的影响是降低复合物的活性和增加膜通透性，但要使 ATP 减少并转化为运动能力降低，需要持续的光照。
