College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China.
Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin, China; The State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China.
Gene. 2022 Jan 10;807:145949. doi: 10.1016/j.gene.2021.145949. Epub 2021 Sep 2.
Growth traits is a critical economic trait for animal husbandry. In this study, the SNPs of CTNNA3 and CAP2 genes were investigated to check whether they are associated with growth traits (body weight, body height, body length and chest circumference) in Hu sheep. The result of the association analysis indicated that the mutation in CTNNA3 (g.2018018 A > G) were associated significantly with body weight, body height, body length and chest circumference (P < 0.05), the mutation in CAP2 (g.8588 T > C) were associated significantly with body height at 140, 160, 180 days (P < 0.05), AA and CC of CTNNA3 and CAP2 were the dominant genotypes associated with growth traits in Hu sheep. Moreover, combined effect analyses indicated that the growth traits with combined genotypes AA-CC and AA-CT were higher than those with genotype GG-CT. RT-qPCR indicated that CTNNA3 expression levels were significantly higher in liver and lung than in other nine tissues (P < 0.05), CAP2 expression levels were significantly higher in bone, heart, liver, lung and duodenum than in other six tissues (P < 0.05). In conclusion, CTNNA3 and CAP2 polymorphisms could be used as genetic markers for improving growth traits in Hu sheep husbandry.
生长性状是畜牧业的一个重要经济性状。本研究旨在探讨 CTNNA3 和 CAP2 基因的 SNP 是否与湖羊的生长性状（体重、体高、体长和胸围）有关。关联分析结果表明，CTNNA3 基因（g.2018018 A>G）突变与体重、体高、体长和胸围显著相关（P<0.05），CAP2 基因（g.8588 T>C）突变与 140、160、180 日龄体高显著相关（P<0.05），AA 和 CC 为 CTNNA3 和 CAP2 与湖羊生长性状相关的优势基因型。此外，联合效应分析表明，AA-CC 和 AA-CT 基因型的生长性状高于 GG-CT 基因型。RT-qPCR 结果表明，CTNNA3 在肝脏和肺部的表达水平显著高于其他 9 种组织（P<0.05），CAP2 在骨骼、心脏、肝脏、肺部和十二指肠中的表达水平显著高于其他 6 种组织（P<0.05）。综上所述，CTNNA3 和 CAP2 多态性可作为湖羊生长性状改良的遗传标记。
