Cheng Jiangbo, Wang Weimin, Zhang Deyin, Zhang Yukun, Li Xiaolong, Zhao Yuan, Xu Dan, Zhao Liming, Li Wenxin, Wang Jianghui, Zhou Bubo, Lin Changchun, Yang Xiaobin, Zhang Xiaoxue
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
The State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Anim Biotechnol. 2023 Dec;34(7):2546-2553. doi: 10.1080/10495398.2022.2105227. Epub 2022 Aug 1.
The aim of this study was to analyze the effect of and genes polymorphisms on growth traits in sheep. The single nucleotide polymorphisms of sheep and genes were identified by DNA sequencing technology. A total of two intronic mutations g.2443 T > C and g.170196 A > G were identified in and , respectively. Correlation analysis was carried out between the obtained genotypes and the growth traits of sheep. The results showed that at the g.2443 T > C locus, the body weight, chest circumference and cannon circumference of the TT genotype sheep were significantly higher than those of the CC genotype sheep ( < .05). At the g.170196 A > G locus, the body weight, body length, chest circumference and cannon circumference of the AA genotype sheep were significantly higher than those of the AG genotype and GG genotype sheep ( < .05). Moreover, the body weight of sheep of combination TT/AA genotype was significantly higher than that of other combination genotypes ( < .05). Therefore, we believe that the polymorphic sites identified in the and genes can be used as candidate molecular markers for breeding good traits in sheep.
本研究旨在分析[基因名称1]和[基因名称2]基因多态性对绵羊生长性状的影响。采用DNA测序技术鉴定绵羊[基因名称1]和[基因名称2]基因的单核苷酸多态性。在[基因名称1]和[基因名称2]中分别共鉴定出两个内含子突变，即g.2443 T＞C和g.170196 A＞G。对获得的基因型与绵羊生长性状进行相关性分析。结果表明，在[基因名称1]的g.2443 T＞C位点，TT基因型绵羊的体重、胸围和管围显著高于CC基因型绵羊（P＜0.05）。在[基因名称2]的g.170196 A＞G位点，AA基因型绵羊的体重、体长、胸围和管围显著高于AG基因型和GG基因型绵羊（P＜0.05）。此外，TT/AA基因型组合的绵羊体重显著高于其他组合基因型（P＜0.05）。因此，我们认为在[基因名称1]和[基因名称2]基因中鉴定出的多态性位点可作为绵羊优良性状选育的候选分子标记。
