College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
The State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu, China.
DNA Cell Biol. 2021 Dec;40(12):1554-1562. doi: 10.1089/dna.2021.0782. Epub 2021 Nov 19.
Herein we detected single nucleotide polymorphisms in and by DNA sequencing and the KASPar technology and analyzed their association with sheep growth traits. Two synonymous mutations, g.1826 C > T and g.10266 G > C, were detected, respectively, and they were found to be significantly associated with sheep growth traits ( < 0.05). In case of g.1826 C > T, the average body weight and chest and cannon circumference of sheep with the CC genotype were significantly higher than those of sheep with the CT and TT genotypes ( < 0.05). Moreover, in case of g.10266 G > C, the average body weight and chest and cannon circumference of sheep with the GG genotype were significantly higher than those of sheep with the GC and CC genotypes ( < 0.05). Moreover, the average body weight of sheep with the CC/GG genotype was higher compared with those of other genotype combinations. We also assessed and expression in different sheep tissues, confirming their expression in all examined tissues. To summarize, we believe that the polymorphisms identified in and can serve as molecular markers for sheep growth traits.
在此，我们通过 DNA 测序和 KASPar 技术检测到 和 中的单核苷酸多态性，并分析了它们与绵羊生长性状的关联。分别检测到两个同义突变，g.1826 C>T 和 g.10266 G>C，发现它们与绵羊生长性状显著相关（<0.05）。对于 g.1826 C>T，CC 基因型绵羊的平均体重、胸围和管围显著高于 CT 和 TT 基因型绵羊（<0.05）。此外，对于 g.10266 G>C，GG 基因型绵羊的平均体重、胸围和管围显著高于 GC 和 CC 基因型绵羊（<0.05）。此外，CC/GG 基因型绵羊的平均体重高于其他基因型组合。我们还评估了不同绵羊组织中 和 的表达，证实它们在所有检查的组织中表达。总之，我们认为 和 中鉴定的多态性可以作为绵羊生长性状的分子标记。
