Ayisi Felix, Mensah Caleb Nketia, Borquaye Lawrence Sheringham
Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Central Laboratory, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
J Parasitol Res. 2021 Aug 29;2021:7347532. doi: 10.1155/2021/7347532. eCollection 2021.
In Africa, medicinal plants are commonly used to treat malaria and other diseased conditions. The ethanolic leaf and twig extract of has been shown to possess promising antiplasmodial properties. This present study was aimed at investigating its antiplasmodial effect . Qualitative phytochemical screening was carried out on the plant samples using standard methods. The antiplasmodial effect against early infection, curative effect against established infection, and prophylactic effect against residual infection were studied in -infected mice while the carrageenan-induced edema model in chicks was used for anti-inflammatory tests. The phosphomolybdenum and DPPH radical scavenging assays were used in the evaluation of antioxidant potential. Acute toxicity of the extract was evaluated using the Organization for Economic Cooperation and Development (OECD) guidelines. Phytochemical screening of plant samples revealed the presence of flavonoids, coumarins, tannins, saponins, and glycosides. leaf and twig extract exhibited significant antiplasmodial activities in the mouse model with parasite suppression rates of 66.63%, 71.70%, and 56.93% in the suppressive, curative, and prophylactic tests, respectively. A 55.50% reduction of edema in the anti-inflammatory test indicated moderate success in reducing inflammation. The total antioxidant capacity of the extract was determined to be 65.4 mg AAE/g of extract, while in the DPPH radical scavenging assay, the IC value was found to be 499.4 g/mL. With the exception of an inconsistent rise in urea level, there was no significant difference in the other biochemistry parameters in the acute toxicity studied. The median lethal dose (LD) of the extract was over 2000 mg/kg. The results of this study show that leaf and twig extract has promising antimalarial capabilities and is fairly safe at low concentrations.
在非洲，药用植物常用于治疗疟疾和其他疾病。已证明[植物名称]的乙醇叶和嫩枝提取物具有良好的抗疟原虫特性。本研究旨在调查其抗疟原虫作用。使用标准方法对植物样品进行了定性植物化学筛选。在感染疟原虫的小鼠中研究了其对早期感染的抗疟原虫作用、对已建立感染的治疗作用以及对残余感染的预防作用，同时使用雏鸡角叉菜胶诱导的水肿模型进行抗炎试验。采用磷钼酸和DPPH自由基清除试验评估抗氧化潜力。根据经济合作与发展组织（OECD）指南评估提取物的急性毒性。植物样品的植物化学筛选显示存在黄酮类、香豆素、单宁、皂苷和糖苷。[植物名称]叶和嫩枝提取物在小鼠模型中表现出显著的抗疟原虫活性，在抑制、治疗和预防试验中的寄生虫抑制率分别为66.63%、71.70%和56.93%。抗炎试验中水肿减少55.50%表明在减轻炎症方面取得了一定成功。提取物的总抗氧化能力测定为65.4毫克抗坏血酸当量/克提取物，而在DPPH自由基清除试验中，IC50值为499.4微克/毫升。在所研究的急性毒性中，除尿素水平出现不一致的升高外，其他生化参数没有显著差异。提取物的半数致死剂量（LD50）超过2000毫克/千克。本研究结果表明，[植物名称]叶和嫩枝提取物具有良好的抗疟能力，在低浓度下相当安全。
