Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi Arabia.
National Research Institute of Rural Engineering, Water and Forestry, University of Tunis Carthage, Street of Hedi Karay BP N 10, Ariana 2080, Tunisia.
Molecules. 2021 Aug 24;26(17):5112. doi: 10.3390/molecules26175112.
The present study aims to evaluate the chemical composition, metabolites secondary and pharmacology activities of methanolic extract of collected from King Saudi Arabia. Moreover, the primary mode of action of the tested extract was studied here for the first time against and HPLC analysis shows that the major components in the tested extract are luteolin-7--d-glucoside, ferulic acid and premarrubiin. Obtained data demonstrated that the investigated extract was richer in phenol (26.8 ± 0.01 mg/GAE g) than in flavonoids (0.61 ± 0.05 mg EC/mL). In addition, the methanolic extract showed an important antioxidant capacity against the DPPH (IC = 35 ± 0.01 µg/mL) and ABTS (IC = 25 ± 0.2 µg/mL) radical scavenging and a strong inhibition of acetylcholinesterase enzyme with an IC value corresponding to 0.4 mg/mL. The antibacterial activity demonstrated that the evaluated extract had significant activity against both Gram-positive and Gram-negative bacteria. The effect of time on cell integrity on and determined by time-kill and bacteriolysis tests showed that the extract reduced the viability of both strains after 8 and 10 h and had a bacteriolytic effect against two different categories of bacteria, Gram-positive and negative, which are not of the same potency. Based on obtained data, it can be concluded that Saudi has a high pharmacological importance and can be used in preparation of food or drugs.
本研究旨在评估从沙特阿拉伯采集的甲醇提取物的化学成分、次生代谢物和药理学活性。此外，这里首次研究了测试提取物对和的主要作用模式。HPLC 分析表明，测试提取物中的主要成分是木樨草素-7--d-葡萄糖苷、阿魏酸和前马里苷。获得的数据表明，所研究的提取物在酚类物质（26.8 ± 0.01 mg/GAE g）方面比类黄酮（0.61 ± 0.05 mg EC/mL）更丰富。此外，该甲醇提取物显示出对 DPPH（IC = 35 ± 0.01 µg/mL）和 ABTS（IC = 25 ± 0.2 µg/mL）自由基清除的重要抗氧化能力，以及对乙酰胆碱酯酶的强烈抑制作用，其 IC 值对应 0.4 mg/mL。抗菌活性表明，评价提取物对革兰氏阳性和革兰氏阴性细菌均具有显著活性。通过时间杀伤和细菌溶解试验确定时间对和细胞完整性的影响表明，提取物在 8 小时和 10 小时后降低了两种菌株的活力，并对革兰氏阳性和阴性两种不同类别的细菌具有溶菌作用，其效力并不相同。基于获得的数据，可以得出结论，沙特阿拉伯具有很高的药理学重要性，可以用于制备食品或药物。
