HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi against Foodborne Diseases Bacteria.

The present study aims to evaluate the chemical composition, metabolites secondary and pharmacology activities of methanolic extract of collected from King Saudi Arabia. Moreover, the primary mode of action of the tested extract was studied here for the first time against and HPLC analysis shows that the major components in the tested extract are luteolin-7--d-glucoside, ferulic acid and premarrubiin. Obtained data demonstrated that the investigated extract was richer in phenol (26.8 ± 0.01 mg/GAE g) than in flavonoids (0.61 ± 0.05 mg EC/mL). In addition, the methanolic extract showed an important antioxidant capacity against the DPPH (IC = 35 ± 0.01 µg/mL) and ABTS (IC = 25 ± 0.2 µg/mL) radical scavenging and a strong inhibition of acetylcholinesterase enzyme with an IC value corresponding to 0.4 mg/mL. The antibacterial activity demonstrated that the evaluated extract had significant activity against both Gram-positive and Gram-negative bacteria. The effect of time on cell integrity on and determined by time-kill and bacteriolysis tests showed that the extract reduced the viability of both strains after 8 and 10 h and had a bacteriolytic effect against two different categories of bacteria, Gram-positive and negative, which are not of the same potency. Based on obtained data, it can be concluded that Saudi has a high pharmacological importance and can be used in preparation of food or drugs.