Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy.
Department of Biomedical and Biotechnological Sciences, Physiology Section, University of Catania, 95123 Catania, Italy.
Molecules. 2021 Sep 5;26(17):5392. doi: 10.3390/molecules26175392.
Osteoarthritis (OA) is a complex disease, source of pain and disability that affects millions of people worldwide. OA etiology is complex, multifactorial and joint-specific, with genetic, biological and biomechanical components. Recently, several studies have suggested a potential adjuvant role for natural extracts on OA progression, in terms of moderating chondrocyte inflammation and following cartilage injury, thus resulting in an overall improvement of joint pain. In this study, we first analyzed the phenylethanoid glycosides profile and the total amount of polyphenols present in a leaf aqueous extract of L. We then investigated the anti-inflammatory and anti-osteoarthritic bioactive potential of the extract in murine monocyte/macrophage-like cells (RAW 264.7) and in human chondrocyte cells (HC), by gene expression analysis of specifics inflammatory cytokines, pro-inflammatory enzymes and metalloproteases. Six phenylethanoid glycosides were identified and the total phenolic content was 124.0 ± 0.7 mg gallic acid equivalent (GAE)/g of extract. The biological investigation showed that the extract is able to significantly decrease most of the cellular inflammatory markers, compared to both control cells and cells treated with (Burch.) DC. ex Meisn, used as a positive control. leaf aqueous extract has the potential to moderate the inflammatory response, representing an innovative possible approach for the inflammatory joint disease treatment.
骨关节炎（OA）是一种复杂的疾病，是导致全球数百万人疼痛和残疾的根源。OA 的病因复杂，具有多因素和关节特异性，包括遗传、生物和生物力学成分。最近，多项研究表明，天然提取物在 OA 进展方面可能具有辅助作用，可调节软骨细胞炎症和软骨损伤后的反应，从而整体改善关节疼痛。在这项研究中，我们首先分析了叶水提物中苯乙醇苷类成分的特征和总多酚含量。然后，我们通过对特定炎症细胞因子、促炎酶和金属蛋白酶的基因表达分析，研究了该提取物在鼠单核细胞/巨噬细胞样细胞（RAW 264.7）和人软骨细胞（HC）中的抗炎和抗 OA 生物活性潜力。鉴定出 6 种苯乙醇苷类成分，总酚含量为 124.0±0.7mg 没食子酸当量（GAE）/g 提取物。生物学研究表明，与对照细胞和阳性对照（Burch.）DC 相比，提取物能显著降低大多数细胞炎症标志物。柳叶菜科植物叶水提物具有调节炎症反应的潜力，为治疗炎症性关节疾病提供了一种创新性的可能方法。
