Serçe Aynur, Toptancı Bircan Çeken, Tanrıkut Sevil Emen, Altaş Sevcan, Kızıl Göksel, Kızıl Süleyman, Kızıl Murat
Chemistry Department, Science Faculty, Dicle University, TR-21280 Diyarbakır, Turkey.
Department of Field Crops, Faculty of Agriculture, Dicle University, TR-21280 Diyarbakır, Turkey.
Food Technol Biotechnol. 2016 Dec;54(4):455-461. doi: 10.17113/ftb.54.04.16.4323.
Antioxidant properties of ethanol extract of (milk thistle) seeds was investigated. We have also investigated the protein damage activated by oxidative Fenton reaction and its prevention by seed extract. Antioxidant potential of seed ethanol extract was measured using different methods, such as lipid peroxidation, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing power assays. The extract significantly decreased DNA damage caused by hydroxyl radicals. Protein damage induced by hydroxyl radicals was also efficiently inhibited, which was confirmed by the presence of protein damage markers, such as protein carbonyl formation and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The present study shows that milk thistle seeds have good DPPH free radical scavenging activity and can prevent lipid peroxidation. Therefore, can be used as potentially rich source of antioxidants and food preservatives. The results suggest that the seeds may have potential beneficial health effects providing opportunities to develop value-added products.
对水飞蓟种子乙醇提取物的抗氧化特性进行了研究。我们还研究了由氧化芬顿反应激活的蛋白质损伤及其被水飞蓟种子提取物的预防作用。使用不同的方法，如脂质过氧化、1,1-二苯基-2-苦基肼（DPPH）和铁还原能力测定法，来测量水飞蓟种子乙醇提取物的抗氧化潜力。该提取物显著降低了由羟基自由基引起的DNA损伤。羟基自由基诱导的蛋白质损伤也得到了有效抑制，这通过蛋白质损伤标记物的存在得到证实，如蛋白质羰基的形成以及十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）。本研究表明，水飞蓟种子具有良好的DPPH自由基清除活性，并且可以防止脂质过氧化。因此，水飞蓟可以用作潜在的丰富抗氧化剂和食品防腐剂来源。结果表明，这些种子可能具有潜在的有益健康作用，为开发增值产品提供了机会。
