Matchynski James I, Manwar Rayyan, Kratkiewicz Karl J, Madangopal Rajtarun, Lennon Veronica A, Makki Kassem M, Reppen Abbey L, Woznicki Alexander R, Hope Bruce T, Perrine Shane A, Conti Alana C, Avanaki Kamran
John D. Dingell Veterans Affairs Medical Center, Detroit, MI, USA.
Translational Neuroscience Program, Wayne State University School of Medicine, Detroit, MI, USA.
Photoacoustics. 2021 Aug 30;24:100297. doi: 10.1016/j.pacs.2021.100297. eCollection 2021 Dec.
Measuring neuroactivity underlying complex behaviors facilitates understanding the microcircuitry that supports these behaviors. We have developed a functional and molecular photoacoustic tomography (F/M-PAT) system which allows direct imaging of Fos-expressing neuronal ensembles in Fos-LacZ transgenic rats with a large field-of-view and high spatial resolution. F/M-PAT measures the beta-galactosidase catalyzed enzymatic product of exogenous chromophore X-gal within ensemble neurons. We used an imaging method in the Wistar Fos-LacZ transgenic rat, to detect neuronal ensembles in medial prefrontal cortex (mPFC) following cocaine administration or a shock-tone paired stimulus. Robust and selective F/M-PAT signal was detected in mPFC neurons after both conditions (compare to naive controls) demonstrating successful and direct detection of Fos-expressing neuronal ensembles using this approach. The results of this study indicate that F/M-PAT can be used in conjunction with Fos-LacZ rats to monitor neuronal ensembles that underlie a range of behavioral processes, such as fear learning or addiction.
测量复杂行为背后的神经活动有助于理解支持这些行为的微电路。我们开发了一种功能和分子光声断层扫描（F/M-PAT）系统，该系统能够在具有大视野和高空间分辨率的Fos-LacZ转基因大鼠中对表达Fos的神经元群进行直接成像。F/M-PAT测量集合神经元中外源发色团X-gal的β-半乳糖苷酶催化的酶产物。我们在Wistar Fos-LacZ转基因大鼠中使用一种成像方法，以检测可卡因给药或电击-音调配对刺激后内侧前额叶皮质（mPFC）中的神经元群。在两种条件下（与未处理的对照相比），均在mPFC神经元中检测到强烈且选择性的F/M-PAT信号，表明使用这种方法成功且直接地检测到了表达Fos的神经元群。这项研究的结果表明，F/M-PAT可与Fos-LacZ大鼠结合使用，以监测一系列行为过程（如恐惧学习或成瘾）背后的神经元群。
