Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, CS, Italy.
Macrofarm s.r.l., c/o Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, CS, Italy.
Nanoscale. 2021 Oct 21;13(40):16885-16899. doi: 10.1039/d1nr03727g.
The present research study reports the development of plastic antibodies based on Molecularly Imprinted Polymers (MIPs) capable of selectively binding a portion of the novel coronavirus SARS-CoV-2 spike protein. Indeed, molecular imprinting represents a very promising and attractive technology for the synthesis of MIPs characterized by specific recognition abilities for a target molecule. Given these characteristics, MIPs can be considered tailor-made synthetic antibodies obtained by a templating process. After analysis, imprinted nanoparticles were synthesized by inverse microemulsion polymerization and their ability to prevent the interaction between ACE2 and the receptor-binding domain of SARS-CoV-2 was investigated. Of relevance, the developed synthetic antibodies are capable of significantly inhibiting virus replication in Vero cell culture, suggesting their potential application in the treatment, prevention and diagnosis of SARS-CoV-2 infection.
本研究报告了基于分子印迹聚合物（MIPs）的塑料抗体的开发，这些抗体能够选择性地结合新型冠状病毒 SARS-CoV-2 刺突蛋白的一部分。事实上，分子印迹技术是一种非常有前途和有吸引力的技术，可用于合成对目标分子具有特定识别能力的 MIPs。鉴于这些特性，MIPs 可以被视为通过模板过程获得的定制合成抗体。经过分析，通过反相微乳液聚合合成了印迹纳米粒子，并研究了它们防止 ACE2 与 SARS-CoV-2 受体结合域相互作用的能力。值得注意的是，所开发的合成抗体能够显著抑制 Vero 细胞培养中的病毒复制，表明它们在 SARS-CoV-2 感染的治疗、预防和诊断中有潜在的应用。
