Li Quan-Quan, Wen Ming-Jie, Zhang Yu-Sen, Guo Zi-Sheng, Bai Xue, Song Jin-Xi, Liu Ping, Wang Yao-Yu, Li Jian-Li
Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, Shaanxi Key Laboratory of Physico-Inorganic Chemistry, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, PR China; Shaanxi Key Laboratory of Earth Surface System and Environmental Carrying Capacity, College of Urban and Environmental Sciences, Northwest University, Xi'an 710127, China.
Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, Shaanxi Key Laboratory of Physico-Inorganic Chemistry, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, PR China.
J Hazard Mater. 2022 Feb 5;423(Pt B):127132. doi: 10.1016/j.jhazmat.2021.127132. Epub 2021 Sep 11.
The abuse of antibiotics has triggered the rise of drug-resistance bacteria, which has seriously threatened public health globally. As a result, carrying out efficient and accurate antibiotic and bacteria identification are quite significant but challenge. Herein, an unprecedented Cd-MOF-based sensor, [CdL] [1, HL = 4-(2-methyl-1H-benzo[d]imidazol-1-yl) isophthalic acid] with multiple fluorescence response behaviours towards antibiotics and bacteria was developed. Single-crystal X-ray diffraction revealed that 1 is a mesomeric 2D bilayer, which is comprised of two opposite chiral mono-layers, each assembled by left-handed or right-handed helixes. More interestingly, 1 represented multiplex detection capability towards antibiotics and bacteria through two detection behaviors: toward nitro-antibiotics and chlortetracycline (CTC) via fluorescent quenching, while toward Staphylococcus albus (S. albus) via fluorescent enhancement. Remarkably, 1 showed a low limit of detection (LOD, 47 CFU/mL) accompanied with specificity in the detection of S. albus compared to other bacteria, such as Staphylococcus aureus, Acinetobacter baumannii, Klebsiella pneumonia, Pseudomonas aeruginosa and Escherichia coli. In addition, the LOD could reach to ppm level for nitro-antibiotics and CTC. Moreover, the practical application of 1 was further reinforced through the detection of nitro-antibiotics and CTC, as well as S. albus in fetal calf serum and river water.
抗生素的滥用引发了耐药菌的增加，这在全球范围内严重威胁着公众健康。因此，开展高效准确的抗生素和细菌鉴定意义重大但颇具挑战。在此，开发了一种前所未有的基于镉金属有机框架（Cd-MOF）的传感器[CdL] [1，HL = 4-(2-甲基-1H-苯并[d]咪唑-1-基)间苯二甲酸]，它对抗生素和细菌具有多种荧光响应行为。单晶X射线衍射表明1是一种介晶二维双层结构，由两个相对的手性单层组成，每个单层由左旋或右旋螺旋组装而成。更有趣的是，1通过两种检测行为表现出对抗生素和细菌的多重检测能力：对硝基抗生素和金霉素（CTC）通过荧光猝灭进行检测，而对白色葡萄球菌（S. albus）通过荧光增强进行检测。值得注意的是，与其他细菌相比，如金黄色葡萄球菌、鲍曼不动杆菌、肺炎克雷伯菌、铜绿假单胞菌和大肠杆菌，1在检测白色葡萄球菌时显示出低检测限（LOD，47 CFU/mL）以及特异性。此外，对硝基抗生素和CTC的检测限可达ppm级别。此外，通过在胎牛血清和河水中检测硝基抗生素、CTC以及白色葡萄球菌，进一步加强了1的实际应用。
