Center for Translational Cancer Research Institute of Biosciences and Technology Texas A&M University Houston TX 77030 USA.
Department of Oral and Maxillofacial Surgery Sun Yat-sen Memorial Hospital Sun Yat-sen University Guangzhou Guangdong 510120 China.
Adv Sci (Weinh). 2021 May 6;8(13):2100424. doi: 10.1002/advs.202100424. eCollection 2021 Jul.
Herein, a set of optogenetic tools (designated LiPOP) that enable photoswitchable necroptosis and pyroptosis in live cells with varying kinetics, is introduced. The LiPOP tools allow reconstruction of the key molecular steps involved in these two non-apoptotic cell death pathways by harnessing the power of light. Further, the use of LiPOPs coupled with upconversion nanoparticles or bioluminescence is demonstrated to achieve wireless optogenetic or chemo-optogenetic killing of cancer cells in multiple mouse tumor models. LiPOPs can trigger necroptotic and pyroptotic cell death in cultured prokaryotic or eukaryotic cells and in living animals, and set the stage for studying the role of non-apoptotic cell death pathways during microbial infection and anti-tumor immunity.
本文介绍了一套光遗传学工具（命名为 LiPOP），可在活细胞中以不同的动力学实现光控坏死和细胞焦亡。LiPOP 工具通过利用光的力量，允许重建这两种非凋亡细胞死亡途径涉及的关键分子步骤。此外，LiPOPs 的使用与上转换纳米粒子或生物发光相结合，被证明可在多种小鼠肿瘤模型中实现无线光遗传学或化学光遗传学杀伤癌细胞。LiPOP 可在培养的原核或真核细胞以及活体动物中引发坏死性和细胞焦亡性细胞死亡，为研究微生物感染和抗肿瘤免疫过程中非凋亡细胞死亡途径的作用奠定了基础。
