Department of Pharmaceutical Technology, Faculty of Pharmacy, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Department of Pharmaceutical Engineering, Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang, 26300, Kuantan, Pahang.
J Biomed Nanotechnol. 2021 Aug 1;17(8):1612-1626. doi: 10.1166/jbn.2021.3122.
Nanotechnology is drawing attention nowadays due to its ability to regulate metals into nanosize, ultimately changing metal's physical, chemical, and optical properties. Silver nanoparticles are known for their potential impact as antimicrobial agents due to their inherent property penetrating the cell wall. The present study aimed to develop and statistically optimise using a novel combination of capsaicin loaded silver nanoparticles (AgCNPs) as an effective anti-bacterial agent to treat psoriasis using a green approach. Ascorbic acid was used as a reducing agent to fabricate silver nanoparticles. The formulation parameters optimisation was conducted using Box-Behnken Design (3×3 factorial design). The loading of capsaicin was confirmed by attenuated total reflectance-fourier transform infrared spectroscopy. Energy-dispersive X-ray spectroscopy-scanning electron microscopy (EDX-SEM) confirmed the existence of silver; net-like structure revealed in SEM and high-resolution transmission electron microscopy further confirmed the nano size of the formulation. Differential scanning calorimetry and X-ray diffraction demonstrated the capsaicin transformed into amorphous after encapsulated. An microbial study showed that the 0.10 M formulation of AgCNPs exerted potent anti-bacterial activity, which can be considered an alternative anti-bacterial agent. It also displayed that the zone of inhibition was significantly high in gram-negative bacteria () than gram-positive bacteria (). Green synthesised AgCNPs showed highly significant anti-bacterial activity, which indicates that this formulation can be very promising for treating psoriasis.
纳米技术因其能够将金属调节到纳米尺寸而引起了人们的关注，从而最终改变金属的物理、化学和光学性质。由于银纳米粒子具有穿透细胞壁的固有特性，因此它们作为抗菌剂具有潜在的影响。本研究旨在开发并通过 Box-Behnken 设计（3×3 析因设计）进行统计学优化，使用辣椒素负载的银纳米粒子（AgCNPs）的新型组合作为一种有效的抗菌剂，以绿色方法治疗银屑病。使用抗坏血酸作为还原剂来制备银纳米粒子。通过衰减全反射-傅里叶变换红外光谱法确认了辣椒素的负载。能量色散 X 射线光谱-扫描电子显微镜（EDX-SEM）证实了银的存在；扫描电子显微镜和高分辨率透射电子显微镜中呈现的网状结构进一步证实了制剂的纳米尺寸。差示扫描量热法和 X 射线衍射表明，包封后辣椒素转化为无定形。微生物研究表明，AgCNPs 的 0.10 M 制剂具有很强的抗菌活性，可被视为替代抗菌剂。它还表明，AgCNPs 的抑菌环在革兰氏阴性菌（）中明显高于革兰氏阳性菌（）。绿色合成的 AgCNPs 表现出高度显著的抗菌活性，这表明该制剂在治疗银屑病方面具有很大的潜力。
