Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, P. O. Box LG 581, Legon, Accra, Ghana.
Vestergaard-NMIMR Vector Labs, Noguchi Memorial Institute for Medical Research, University of Ghana, P. O. Box LG 581, Legon, Accra, Ghana.
Sci Rep. 2021 Sep 20;11(1):18658. doi: 10.1038/s41598-021-98268-2.
A vertically transmitted microsporidian, Microsporidia MB, with the ability to disrupt Plasmodium development was reported in Anopheles arabiensis from Kenya, East Africa. To demonstrate its range of incidence, archived DNA samples from 7575 Anopheles mosquitoes collected from Ghana were screened. MB prevalence was observed at 1.8%. An. gambiae s.s constituted 87% of positive mosquitoes while the remaining were from An. coluzzii. Both sibling species had similar positivity rates (24% and 19%; p = 0.42) despite the significantly higher number of An. gambiae s.s analysed (An. gambiae s.s = 487; An. coluzzii = 94; p = 0.0005). The microsporidian was also more prevalent in emerged adults from field-collected larvae than field-caught adults (p < 0.0001) suggestive of an efficient vertical transmission and/or horizontal transfer among larvae. This is the first report of Microsporidia MB in Anopheles mosquitoes in West Africa. It indicates possible widespread among malaria vector species and warrants investigations into the symbiont's diversity across sub-Saharan Africa.
在东非肯尼亚的阿拉伯按蚊中发现了一种垂直传播的微孢子虫 Microsporidia MB，它具有破坏疟原虫发育的能力。为了证明其发病范围，对加纳收集的 7575 只按蚊的存档 DNA 样本进行了筛查。MB 的患病率为 1.8%。阳性蚊子中 87%为冈比亚按蚊 s.s，其余来自库蚊。尽管分析的冈比亚按蚊 s.s 数量明显更多（冈比亚按蚊 s.s=487；库蚊=94；p=0.0005），但两种姊妹种的阳性率相似（24%和 19%；p=0.42）。微孢子虫在野外收集的幼虫中羽化的成虫中也更为普遍，而在野外捕获的成虫中则较少（p<0.0001），这表明垂直传播和/或幼虫之间的水平传播效率很高。这是 Microsporidia MB 在西非按蚊中的首次报告。它表明该共生体可能在疟疾传播媒介物种中广泛存在，值得对撒哈拉以南非洲的共生体多样性进行调查。
Zotero 插件