NEST & NPOED Lab, Department of Physics, Cochin University of Science and Technology, Kochi, 682022, India.
Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Kochi, 682022, India.
J Fluoresc. 2021 Nov;31(6):1927-1936. doi: 10.1007/s10895-021-02814-0. Epub 2021 Sep 21.
Hen's eggshell, a biological waste product, was turned into a cell imaging probe: europium doped hydroxyapatite (HAp: Eu) nanoparticle using hydrothermal method. Luminescence of the synthesized nanoparticle was studied for various doping concentrations of the lanthanide ion europium (Eu). Eu doped HAp showed a hexagonal crystal structure and rod-shaped morphology. Well-defined emission peaks of europium, corresponding to the substitution of Eu at the Ca(I) site of HAp, were confirmed from the samples' photoluminescence (PL) spectra. Good biocompatibility up to 500 μg/mL of the samples indicates their potential applications in bioimaging. Synthesized nanoparticles were internalized and used for in vitro imaging of the PC12 cells without any surface modification. The materials' use as a potential in vivo imaging agent is proposed from the haemolysis study.
鸡蛋壳是一种生物废弃物，通过水热法将其转化为细胞成像探针：掺铕羟基磷灰石（HAp：Eu）纳米粒子。研究了合成纳米粒子在不同镧系元素铕（Eu）掺杂浓度下的发光性能。Eu 掺杂的 HAp 呈现出六方晶相结构和棒状形态。从样品的光致发光（PL）光谱中证实了 Eu 的特征发射峰，这对应于 Eu 在 HAp 的 Ca（I）位的取代。样品的细胞毒性实验表明，其在 500μg/mL 浓度下具有良好的生物相容性，这表明它们在生物成像方面具有潜在的应用。合成的纳米粒子无需表面修饰即可被内化并用于 PC12 细胞的体外成像。从溶血研究中提出了将这些材料用作潜在的体内成像剂的可能性。
