Jankowska-Kieltyka Monika, Roman Adam, Mikrut Magdalena, Kowalska Marta, van Eldik Rudi, Nalepa Irena
Department of Brain Biochemistry, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna Street 12, 31-343 Kraków, Poland.
Faculty of Chemistry, Jagiellonian University, Gronostajowa Street 2, 30-387 Kraków, Poland.
Toxics. 2021 Aug 30;9(9):205. doi: 10.3390/toxics9090205.
Exposure to air pollution from various airborne particulate matter (PM) is regarded as a potential health risk. Airborne PM penetrates the lungs, where it is taken up by macrophages, what results in macrophage activation and can potentially lead to negative consequences for the organism. In the present study, we assessed the effects of direct exposure of RAW 264.7 macrophages to crude PM (NIST1648a) and to a reduced content of organic matter (LAp120) for up to 72 h on selected parameters of metabolic activity. These included cell viability and apoptosis, metabolic activity and cell number, ROS synthesis, nitric oxide (NO) release, and oxidative burst. The results indicated that both NIST1648a and LAp120 negatively influenced the parameters of cell viability and metabolic activity due to increased ROS synthesis. The negative effect of PM was concentration-dependent; i.e., it was the most pronounced for the highest concentration applied. The impact of PM also depended on the time of exposure, so at respective time points, PM induced different effects. There were also differences in the impact of NIST1648a and LAp120 on almost all parameters tested. The negative effect of LAp120 was more pronounced, what appeared to be associated with an increased content of metals.
暴露于各种空气中的颗粒物（PM）所造成的空气污染被视为一种潜在的健康风险。空气中的PM会穿透肺部，在肺部被巨噬细胞摄取，这会导致巨噬细胞活化，并可能对机体产生负面影响。在本研究中，我们评估了将RAW 264.7巨噬细胞直接暴露于粗颗粒物（NIST1648a）和有机物含量降低的颗粒物（LAp120）长达72小时对选定代谢活性参数的影响。这些参数包括细胞活力和凋亡、代谢活性和细胞数量、活性氧（ROS）合成、一氧化氮（NO）释放以及氧化爆发。结果表明，由于ROS合成增加，NIST1648a和LAp120均对细胞活力和代谢活性参数产生负面影响。PM的负面影响具有浓度依赖性；即，在所应用的最高浓度下最为明显。PM的影响还取决于暴露时间，因此在各个时间点，PM会产生不同的影响。NIST1648a和LAp120对几乎所有测试参数的影响也存在差异。LAp120的负面影响更为明显，这似乎与金属含量增加有关。
