Alarcón-Apablaza Josefa, Godoy-Sánchez Karina, Jarpa-Parra Marcela, Garrido-Miranda Karla, Fuentes Ramón
Doctoral Program in Morphological Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco 4780000, Chile.
Research Center in Dental Sciences (CICO-UFRO), Dental School, Faculty of Dentistry, Universidad de La Frontera, Temuco 4780000, Chile.
Polymers (Basel). 2024 Dec 16;16(24):3499. doi: 10.3390/polym16243499.
(1) Background: Collagen, a natural polymer, is widely used in the fabrication of membranes for guided bone regeneration (GBR). These membranes are sourced from various tissues, such as skin, pericardium, peritoneum, and tendons, which exhibit differences in regenerative outcomes. Therefore, this study aimed to evaluate the morphological and chemical properties of porcine collagen membranes from five different tissue sources: skin, pericardium, dermis, tendons, and peritoneum. (2) Methods: The membrane structure was analyzed using energy-dispersive X-ray spectrometry (EDX), variable pressure scanning electron microscopy (VP-SEM), Fourier transform infrared spectroscopy (FTIR), and thermal stability via thermogravimetric analysis (TGA). The absorption capacity of the membranes for GBR was also assessed using an analytical digital balance. (3) Results: The membranes displayed distinct microstructural features. Skin- and tendon-derived membranes had rough surfaces with nanopores (1.44 ± 1.24 µm and 0.46 ± 0.1 µm, respectively), while pericardium- and dermis-derived membranes exhibited rough surfaces with macropores (78.90 ± 75.89 µm and 64.89 ± 13.15 µm, respectively). The peritoneum-derived membrane featured a rough surface of compact longitudinal fibers with irregular macropores (9.02 ± 3.70 µm). The thickness varied significantly among the membranes, showing differences in absorption capacity. The pericardium membrane exhibited the highest absorption, increasing by more than 10 times its initial mass. In contrast, the skin-derived membrane demonstrated the lowest absorption, increasing by less than 4 times its initial mass. Chemical analysis revealed that all membranes were primarily composed of carbon, nitrogen, and oxygen. Thermogravimetric and differential scanning calorimetry analyses showed no significant compositional differences among the membranes. FTIR spectra confirmed the presence of collagen, with characteristic peaks corresponding to Amide A, B, I, II, and III. (4) Conclusions: The tissue origin of collagen membranes significantly influences their morphological characteristics, which may, in turn, affect their osteogenic properties. These findings provide valuable insights into the selection of collagen membranes for GBR applications.
(1) 背景：胶原蛋白作为一种天然聚合物，被广泛用于制备引导骨再生（GBR）膜。这些膜来源于各种组织，如皮肤、心包、腹膜和肌腱，它们在再生效果上存在差异。因此，本研究旨在评估来自五种不同组织来源（皮肤、心包、真皮、肌腱和腹膜）的猪胶原蛋白膜的形态和化学性质。(2) 方法：使用能量色散X射线光谱法（EDX）、可变压力扫描电子显微镜（VP - SEM）、傅里叶变换红外光谱法（FTIR）以及通过热重分析（TGA）来分析膜的热稳定性。还使用分析数字天平评估了这些膜对GBR的吸收能力。(3) 结果：这些膜呈现出不同的微观结构特征。来源于皮肤和肌腱的膜表面粗糙，带有纳米孔（分别为1.44±1.24微米和0.46±0.1微米），而来源于心包和真皮的膜表面粗糙，带有大孔（分别为78.90±75.89微米和64.89±13.15微米）。来源于腹膜的膜具有紧密纵向纤维的粗糙表面，带有不规则大孔（9.02±3.70微米）。膜的厚度在各膜之间有显著差异，吸收能力也有所不同。心包膜的吸收能力最高，增加量超过其初始质量的10倍。相比之下，来源于皮肤的膜吸收能力最低，增加量不到其初始质量的4倍。化学分析表明，所有膜主要由碳、氮和氧组成。热重分析和差示扫描量热法分析表明，各膜之间在成分上没有显著差异。FTIR光谱证实了胶原蛋白的存在，具有对应于酰胺A、B、I、II和III的特征峰。(4) 结论：胶原蛋白膜的组织来源显著影响其形态特征，进而可能影响其成骨特性。这些发现为GBR应用中胶原蛋白膜的选择提供了有价值的见解。
