Guangdong Academy of Forestry, Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangzhou 510520, China.
Genes (Basel). 2023 Jul 8;14(7):1416. doi: 10.3390/genes14071416.
NAC proteins play an essential role in the growth and development of litchi, especially during reproductive development. However, a comprehensive analysis of the litchi gene family is currently absent. Based on information from the litchi genome, we found that the 112 NAC genes of litchi show an uneven distribution on the chromosomes. Phylogenetic and conserved structural domain analyses indicated that different types of variability were exhibited in the family of litchi NACs (LcNACs). Gene covariance analysis showed that the showed better similarity in the same genus than with . We further investigated the differential expression patterns of in buds and rudimentary leaves of litchi. qRT-PCR results implied that they were involved in the process. Profiling of promoter elements in litchi showed that they were extensively involved in light response, phytohormone regulation, abiotic stress response, and plant growth and development processes. This study provides new insights into the identification, structural characterization, tissue-specific expression analysis, and promoter response elements of . It reveals the characteristics of the and lays the foundation for the subsequent understanding of its biological functions and molecular regulatory mechanisms.
NAC 蛋白在荔枝的生长发育中起着至关重要的作用，特别是在生殖发育过程中。然而，目前对荔枝基因家族的综合分析还不存在。基于荔枝基因组的信息，我们发现荔枝的 112 个 NAC 基因在染色体上呈现不均匀分布。系统发生和保守结构域分析表明，荔枝 NAC 家族（LcNACs）表现出不同类型的可变性。基因共线性分析表明，在同一属中，不同类型的基因表现出更好的相似性。我们进一步研究了在荔枝芽和原基叶中 的差异表达模式。qRT-PCR 结果表明它们参与了这一过程。荔枝 启动子元件的分析表明，它们广泛参与光反应、植物激素调节、非生物胁迫响应以及植物生长发育过程。本研究为荔枝中的鉴定、结构特征、组织特异性表达分析以及启动子响应元件提供了新的见解。它揭示了 的特征，并为随后理解其生物学功能和分子调控机制奠定了基础。
