Liang Youling, Yan Bin, Meng Zhishang, Xie Manyun, Liang Zhou, Zhu Ziyi, Meng Yongan, Ma Jiayue, Ma Bosheng, Yao Xiaoxi, Luo Jing
Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Shenzhen College of International Education, Shenzhen, China.
Front Med (Lausanne). 2021 Sep 8;8:699254. doi: 10.3389/fmed.2021.699254. eCollection 2021.
To compare the aqueous concentrations of inflammatory and angiogenetic factors in vitrectomized vs. non-vitrectomized eyes with diabetic macular edema (DME). Aqueous samples were obtained from 107 eyes with DME before intravitreal injection of anti-VEGF, 36 eyes with previous pars plana vitrectomy (PPV) combined with pan-retinal endolaser photocoagulation (PRP), and 71 treatment-naïve. Interleukin (IL)-6, IL-8, interferon-induced protein (IP)-10, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF) were measured by cytometric bead array (CBA). Optical coherence tomography (OCT) was used for measuring central retinal thickness (CRT). IL-6, IL-8, IP-10, and MCP-1 in aqueous humor of DME vitrectomized eyes were significantly higher than in non-vitrectomized DME eyes, while VEGF was lower than in non-vitrectomized DME eyes. VEGF in aqueous humor significantly correlated with CRT for DME in non-vitrectomized DME eyes. IL-6, IL-8, IP-10, and MCP-1 in aqueous humor were not significantly associated with VEGF for DME in vitrectomized eyes. Inflammation might play an important role in the pathogenesis of DME in vitrectomized eyes. Moreover, inflammation might play a central role in the development of DME the VEGF-independent pathway. Thus, anti-inflammatory therapy might be a strategy for DME in vitrectomized eyes.
比较接受玻璃体切割术与未接受玻璃体切割术的糖尿病性黄斑水肿（DME）患眼房水中炎症因子和血管生成因子的浓度。在玻璃体内注射抗血管内皮生长因子（VEGF）之前，从107例DME患眼中获取房水样本，其中36例曾行玻璃体切割联合全视网膜激光光凝术（PPV），71例为初治患者。通过细胞计数珠阵列（CBA）检测白细胞介素（IL）-6、IL-8、干扰素诱导蛋白（IP）-10、单核细胞趋化蛋白（MCP）-1和血管内皮生长因子（VEGF）。使用光学相干断层扫描（OCT）测量中心视网膜厚度（CRT）。接受玻璃体切割术的DME患眼房水中的IL-6、IL-8、IP-10和MCP-1显著高于未接受玻璃体切割术的DME患眼，而VEGF则低于未接受玻璃体切割术的DME患眼。未接受玻璃体切割术的DME患眼房水中的VEGF与CRT显著相关。接受玻璃体切割术的DME患眼房水中的IL-6、IL-8、IP-10和MCP-1与VEGF无显著相关性。炎症可能在接受玻璃体切割术的DME患眼的发病机制中起重要作用。此外，炎症可能在DME的发展中通过VEGF非依赖途径起核心作用。因此，抗炎治疗可能是治疗接受玻璃体切割术的DME患眼的一种策略。
