Tertiş Mihaela, Florea Anca, Adumitrăchioaie Alina, Cernat Andreea, Bogdan Diana, Barbu-Tudoran Lucian, Jaffrezic Renault Nicole, Săndulescu Robert, Cristea Cecilia
Analytical Chemistry Department, Iuliu Haţieganu University of Medicine and Pharmacy, 4 Louis Pasteur St, 400349, Cluj-Napoca, Romania.
National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat St, 400293, Cluj-Napoca, Romania.
Chempluschem. 2017 Apr;82(4):561-569. doi: 10.1002/cplu.201600539. Epub 2016 Dec 13.
A new biomimetic electrochemical sensor was developed for the detection of dopamine based on a glassy carbon electrode modified with electrochemically generated gold nanoparticles. The preparation of the polymer is simple and cost-effective, achieving the polymerization of thioaniline and generation of gold nanoparticles in a single step by cyclic voltammetry, in the presence of the target molecule, dopamine. After extraction, the imprinted polymer exhibits high sensitivity and selectivity for dopamine. Moreover, the developed imprinted polymer film allows the fast, direct detection of dopamine without the need of a redox mediator. The formation of a self-assembled monolayer of the monomer prior to electropolymerization ensures the adherence of the film onto the electrode surface, conferring good stability to the sensor (over two weeks). Cyclic voltammetry, electrochemical impedance spectroscopy, atomic force microscopy, scanning electron microscopy, and energy dispersive X-ray spectroscopy were used for the complete characterization of the developed sensor, and differential pulse voltammetry was used for its testing.
基于电化学合成金纳米粒子修饰的玻碳电极，开发了一种用于检测多巴胺的新型仿生电化学传感器。该聚合物的制备简单且具有成本效益，通过循环伏安法在目标分子多巴胺存在的情况下，一步实现硫代苯胺的聚合和金纳米粒子的生成。萃取后，印迹聚合物对多巴胺表现出高灵敏度和选择性。此外，所开发的印迹聚合物膜允许快速、直接检测多巴胺，而无需氧化还原介质。在电聚合之前形成单体的自组装单分子层可确保膜附着在电极表面，赋予传感器良好的稳定性（超过两周）。使用循环伏安法、电化学阻抗谱、原子力显微镜、扫描电子显微镜和能量色散X射线光谱对所开发的传感器进行全面表征，并使用差分脉冲伏安法对其进行测试。
