Kim Ki-Tae, Kim Jin-Woo, Kim Sun-Il, Kim Seonyoung, Nguyen Trung Hau, Kang Chang-Ho
MEDIOGEN, Co., Ltd., Bio Valley 1-ro, Jecheon-si 27159, Korea.
Microorganisms. 2021 Sep 16;9(9):1971. doi: 10.3390/microorganisms9091971.
Oxidative stress is a phenomenon caused by an imbalance between the production of reactive oxygen species and antioxidant defenses. It plays an important role in numerous disease states, including chronic kidney disease, neurological disorders, cardiovascular diseases, diabetes, and cancer. Lactic acid bacteria (LAB) are known to have prominent antioxidant properties. Therefore, this study aimed to measure the antioxidant activity and anti-inflammatory potential of LAB isolated from animals for the efficient use of probiotics with host specificity. Antioxidant activity measurements of sixteen strains revealed that ABTS radical scavenging activities ranged from 26.3 to 57.4%, and DPPH free radical scavenging activities ranged from 4.7 to 13.5%. Based on the antioxidant activity assessment, five strains ( MG9003(YH9003) MG9007(YH9007), MG9012(YH9012), MG9014(YH9014), and MG9015(YH9015)) were selected with the consideration of fermentation productivity (>1 × 10 CFU/g). The selected strains exhibited nitric oxide inhibition and inhibited inducible nitric oxide synthase and cyclooxygenase expression. Furthermore, probiotic properties, including intestinal adhesion and stability, were identified. Our results show that the selected animal-derived strains can be effective probiotic candidates for potential effects on animal hosts.
氧化应激是一种由活性氧生成与抗氧化防御之间失衡所引起的现象。它在众多疾病状态中发挥着重要作用，包括慢性肾脏病、神经紊乱、心血管疾病、糖尿病和癌症。已知乳酸菌（LAB）具有显著的抗氧化特性。因此，本研究旨在测定从动物中分离出的乳酸菌的抗氧化活性和抗炎潜力，以便高效利用具有宿主特异性的益生菌。对16株菌株的抗氧化活性测定表明，ABTS自由基清除活性范围为26.3%至57.4%，DPPH自由基清除活性范围为4.7%至13.5%。基于抗氧化活性评估，考虑到发酵生产力（>1×10 CFU/g），选择了5株菌株（MG9003(YH9003)、MG9007(YH9007)、MG9012(YH9012)、MG9014(YH9014)和MG9015(YH9015)）。所选菌株表现出一氧化氮抑制作用，并抑制诱导型一氧化氮合酶和环氧化酶的表达。此外，还鉴定了包括肠道黏附性和稳定性在内的益生菌特性。我们的结果表明，所选的动物源菌株可能是对动物宿主具有潜在作用的有效益生菌候选菌株。
