Manouras Theodore, Platania Varvara, Georgopoulou Anthie, Chatzinikolaidou Maria, Vamvakaki Maria
Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas, 700 13 Heraklion, Greece.
Department of Materials Science and Technology, University of Crete, 700 13 Heraklion, Greece.
Polymers (Basel). 2021 Sep 9;13(18):3051. doi: 10.3390/polym13183051.
In this work, the antimicrobial action of partially quaternized poly(2-(dimethylamino)ethyl methacrylate) (PQDMAEMA) copolymers using different alkyl halides is presented. The poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) homopolymer was synthesized by group transfer polymerization, followed by the modification of its tertiary amine groups, using bromoethane, iodoethane, bromohexane and bromoethanol, to introduce permanent cationic, quaternary ammonium salt moieties, randomly distributed along the polymer chains. In all cases, the degree of quaternization was low, at ~10 mol%, as verified by proton nuclear magnetic resonance spectroscopy to preserve the thermo-responsive character of the PDMAEMA precursor polymer. The biocidal activity of the lightly quaternized PQDMAEMA copolymers against and was evaluated by calculating the minimum inhibitory concentration (MIC) as well as the minimum bactericidal concentration (MBC) of the polymers and by comparing them to the respective values of the precursor non-quaternized PDMAEMA homopolymer. The antibacterial mechanism of action in the solution was studied by zeta potential measurements, scanning electron microscopy and protein leakage tests signifying the disruption of the outer membrane of the bacterial cells to release their periplasmic proteins.
在本研究中，展示了使用不同卤代烷对部分季铵化的聚甲基丙烯酸2-(二甲基氨基)乙酯(PQDMAEMA)共聚物的抗菌作用。聚甲基丙烯酸2-(二甲基氨基)乙酯(PDMAEMA)均聚物通过基团转移聚合合成，随后使用溴乙烷、碘乙烷、溴己烷和溴乙醇对其叔胺基团进行改性，以引入沿聚合物链随机分布的永久性阳离子季铵盐部分。在所有情况下，季铵化程度都很低，约为10 mol%，这通过质子核磁共振光谱得到验证，以保留PDMAEMA前体聚合物的热响应特性。通过计算聚合物的最低抑菌浓度(MIC)以及最低杀菌浓度(MBC)，并将它们与前体未季铵化的PDMAEMA均聚物的相应值进行比较，评估了轻度季铵化的PQDMAEMA共聚物对[具体细菌名称1]和[具体细菌名称2]的杀菌活性。通过zeta电位测量、扫描电子显微镜和蛋白质泄漏试验研究了溶液中的抗菌作用机制，这些试验表明细菌细胞外膜被破坏，从而释放其周质蛋白。
