Pahlevanneshan Zari, Deypour Mohammadreza, Kefayat Amirhosein, Rafienia Mohammad, Sajkiewicz Paweł, Esmaeely Neisiany Rasoul, Enayati Mohammad Saeid
Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Medical Technologies, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
Polymer Chemistry Research Laboratory, Department of Chemistry, Isfahan 81746-73441, Iran.
Polymers (Basel). 2021 Sep 20;13(18):3191. doi: 10.3390/polym13183191.
This piece of research explores porous nanocomposite polyurethane (PU) foam synthesis, containing nanolignin (NL), coated with natural antimicrobial propolis for wound dressing. PU foam was synthesized using polyethylene glycol, glycerol, NL, and 1, 6-diisocyanato-hexane (NCO/OH ratio: 1.2) and water as blowing agent. The resultant foam was immersed in ethanolic extract of propolis (EEP). PU, NL-PU, and PU-NL/EEP foams were characterized from mechanical, morphological, and chemical perspectives. NL Incorporation into PU increased mechanical strength, while EEP coating showed lower strength than PU-NL/EEP. Morphological investigations confirmed an open-celled structure with a pore diameter of 150-200 μm, a density of nearly 0.2 g/cm, and porosity greater than 85%, which led to significantly high water absorption (267% for PU-NL/EEP). The hydrophilic nature of foams, measured by the contact angle, proved to be increased by NL addition and EEP coating. PU and PU-NL did not show important antibacterial features, while EEP coating resulted in a significant antibacterial efficiency. All foams revealed high biocompatibility toward L929 fibroblasts, with the highest cell viability and cell attachment for PU-NL/EEP. In vivo wound healing using Wistar rats' full-thickness skin wound model confirmed that PU-NL/EEP exhibited an essentially higher wound healing efficacy compared with other foams. Hence, PU-NL/EEP foam could be a promising wound dressing candidate.
本研究探索了一种用于伤口敷料的多孔纳米复合聚氨酯（PU）泡沫的合成方法，该泡沫含有纳米木质素（NL），并涂覆有天然抗菌剂蜂胶。PU泡沫是使用聚乙二醇、甘油、NL和1,6 - 二异氰酸己酯（NCO/OH比例为1.2）以及水作为发泡剂合成的。所得泡沫浸泡在蜂胶乙醇提取物（EEP）中。从力学、形态和化学角度对PU、NL - PU和PU - NL/EEP泡沫进行了表征。将NL掺入PU中提高了机械强度，而EEP涂层的强度低于PU - NL/EEP。形态学研究证实其具有开孔结构，孔径为150 - 200μm，密度接近0.2g/cm，孔隙率大于85%，这导致吸水率显著较高（PU - NL/EEP为267%）。通过接触角测量发现，添加NL和EEP涂层可提高泡沫的亲水性。PU和PU - NL没有表现出重要的抗菌特性，而EEP涂层具有显著的抗菌效率。所有泡沫对L929成纤维细胞均显示出高生物相容性，其中PU - NL/EEP的细胞活力和细胞附着率最高。使用Wistar大鼠全层皮肤伤口模型进行的体内伤口愈合实验证实，与其他泡沫相比，PU - NL/EEP具有更高的伤口愈合效果。因此，PU - NL/EEP泡沫可能是一种有前景的伤口敷料候选材料。
