Beddiar Hatem, Boudiba Sameh, Benahmed Merzoug, Tamfu Alfred Ngenge, Ceylan Özgür, Hanini Karima, Kucukaydin Selcuk, Elomri Abdelhakim, Bensouici Chawki, Laouer Hocine, Akkal Salah, Boudiba Louiza, Dinica Rodica Mihaela
Laboratory of Organic Materials and Heterochemistry, Tebessa University, Constantine Road, Tebessa 12002, Algeria.
Laboratory of Bioactive Molecules and Applications, Tebessa University, Constantine Road, Tebessa 12002, Algeria.
Plants (Basel). 2021 Sep 18;10(9):1955. doi: 10.3390/plants10091955.
Phenolic extracts of were prepared and their preliminary phenolic profiles determined using HPLC-DAD with 26 phenolic standards. Apigenin (21.75 ± 0.41 µg/g), myricetin (72.58 ± 0.57 µg/g), and rosmarinic acid (88.51 ± 0.55 µg/g) were the most abundant compounds in DCM (dichloromethane), AcOEt (ethyl acetate), and BuOH (butanol) extracts, respectively. The DCM and AcOEt extracts inhibited quorum-sensing mediated violacein production by CV12472. Anti-quorum-sensing zones on CV026 at MIC (minimal inhibitory concentration) were 10.3 ± 0.8 mm for DCM extract and 12.0 ± 0.5 mm for AcOEt extract. Extracts showed concentration-dependent inhibition of swarming motility on flagellated PA01 and at the highest test concentration of 100 μg/mL, AcOEt (35.42 ± 1.00%) extract displayed the best activity. FRAP assay indicated that the BuOH extract (A = 17.42 ± 0.25 µg/mL) was more active than standard α-tocopherol (A = 34.93 ± 2.38 µg/mL). BuOH extract was more active than other extracts except in the ABTS, where the DCM extract was most active. This antioxidant activity could be attributed to the phenolic compounds detected. extracts showed moderate inhibition on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, and α-amylase. The results indicate that is a potent source of natural antioxidants that could be used in managing microbial resistance and Alzheimer's disease.
制备了[具体物质]的酚类提取物，并使用配备26种酚类标准品的高效液相色谱-二极管阵列检测器（HPLC-DAD）测定了其初步酚类概况。芹菜素（21.75±0.41微克/克）、杨梅素（72.58±0.57微克/克）和迷迭香酸（88.51±0.55微克/克）分别是二氯甲烷（DCM）、乙酸乙酯（AcOEt）和丁醇（BuOH）提取物中含量最丰富的化合物。DCM和AcOEt提取物抑制了CV12472群体感应介导的紫色杆菌素产生。在最小抑菌浓度（MIC）下，DCM提取物对CV026的群体感应抑制圈为10.3±0.8毫米，AcOEt提取物为12.0±0.5毫米。提取物对鞭毛型铜绿假单胞菌PA01的群游运动表现出浓度依赖性抑制，在最高测试浓度100微克/毫升时，AcOEt提取物（35.42±1.00%）表现出最佳活性。铁离子还原抗氧化能力（FRAP）测定表明，BuOH提取物（吸光度A = 17.42±0.25微克/毫升）比标准α-生育酚（吸光度A = 34.93±2.38微克/毫升）更具活性。除了在ABTS测定中DCM提取物活性最高外，BuOH提取物比其他提取物更具活性。这种抗氧化活性可能归因于检测到的酚类化合物。[具体物质]提取物对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、酪氨酸酶和α-淀粉酶表现出适度抑制。结果表明，[具体物质]是天然抗氧化剂的有力来源，可用于应对微生物耐药性和阿尔茨海默病。
