Ceylan Ozgur, Tamfu Alfred Ngenge, Doğaç Yasemin İspirli, Teke Mustafa
Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Mugla Sitki Kocman University, Mugla, Turkey.
Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, 454, Ngaoundere, Cameroon.
3 Biotech. 2020 Dec;10(12):513. doi: 10.1007/s13205-020-02509-6. Epub 2020 Nov 7.
This study was aimed at synthesizing polyethyleneimine-coated magnetic nanoparticles and evaluating their effect on pathogenic bacteria. Polyethyleneimine-coated magnetite (PEIMnF) and nickel ferrite (PEINF) nanoparticles were succesfully synthesized and their surface groups, morphology and chemical structures were characterized using ATR-FTIR (Attenuated Total Reflectance Fourrier Transformed Infra-Red) and SEM (Scanning Electron Microscopy). TGA (Thermogravimetric analysis) was used to analyse the thermal behaviour and stability of synthesized nanomaterials. The minimal inhibitory concentration (MIC) values of the polyethylene imine coated magnetite and nickel ferrite nanomaterials against , and was found to be 10 mg/mL. Both nanomaterials (PEIMnF and PEINF) showed very excellent and concentration-dependent biofilm inhibition especially at the highest test concentration of 10 mg/mL at which PEIMnF inhibited biofilm formation on (89.04 ± 0.50%), (82.85 ± 2.42%) and (91.37 ± 0.66%). At this concentration, PEINF equally inhibited biofilm formations of (90.48 ± 2.05%), (87.04 ± 1.59%) and (90.94 ± 1.03%). Only PEINF showed a concentration-dependent violacein inhibition with highest inhibition of 51.2 ± 3.5% at MIC and quorum sensing with inhibition zones of 16.3 ± 1.0 mm at MIC and 11.5 ± 0.5 mm at MIC/2 which could be attributed to the presence of nickel. The nanomaterials inhibited swimming and swarming motilities in PA01 and it was found that at the same concentration, swimming inhibition was greater than swarming inhibitions and PEINF showed better inhibition than PEIMnF in both models. Polyethyleneimine-coated magnetite and nickel ferrite nanomaterials could be used in overcoming health problems associated with microbial infections and resistance.
本研究旨在合成聚乙烯亚胺包覆的磁性纳米颗粒，并评估其对病原菌的作用。成功合成了聚乙烯亚胺包覆的磁铁矿（PEIMnF）和镍铁氧体（PEINF）纳米颗粒，并使用衰减全反射傅里叶变换红外光谱（ATR-FTIR）和扫描电子显微镜（SEM）对其表面基团、形态和化学结构进行了表征。热重分析（TGA）用于分析合成纳米材料的热行为和稳定性。发现聚乙烯亚胺包覆的磁铁矿和镍铁氧体纳米材料对大肠杆菌、金黄色葡萄球菌和白色念珠菌的最小抑菌浓度（MIC）值为10mg/mL。两种纳米材料（PEIMnF和PEINF）均表现出非常优异的、浓度依赖性的生物膜抑制作用，尤其是在最高测试浓度10mg/mL时，PEIMnF对铜绿假单胞菌（89.04±0.50%）、金黄色葡萄球菌（82.85±2.42%）和白色念珠菌（91.37±0.66%）的生物膜形成具有抑制作用。在此浓度下，PEINF同样对铜绿假单胞菌（90.48±2.05%）、金黄色葡萄球菌（87.04±1.59%）和白色念珠菌（90.94±1.03%）的生物膜形成具有抑制作用。只有PEINF表现出浓度依赖性的紫菌素抑制作用，在MIC时最高抑制率为51.2±3.5%，群体感应在MIC时抑制圈为16.3±1.0mm，在MIC/2时为11.5±0.5mm，这可能归因于镍的存在。纳米材料抑制了铜绿假单胞菌PA01的游泳和群集运动，发现在相同浓度下，游泳抑制作用大于群集抑制作用，并且在两种模型中PEINF的抑制作用均优于PEIMnF。聚乙烯亚胺包覆的磁铁矿和镍铁氧体纳米材料可用于克服与微生物感染和耐药性相关的健康问题。
