School of Dentistry, University of Missouri - Kansas City, Kansas City, MO, 64108, USA.
School of Dentistry, University of Missouri - Kansas City, Kansas City, MO, 64108, USA.
Dent Mater. 2021 Dec;37(12):1794-1805. doi: 10.1016/j.dental.2021.09.009. Epub 2021 Sep 25.
To evaluate the effects of different polyphenols and solvents on dentin collagen's crosslinking interactions and biostabilization against MMPs and collagenase degradation.
Two polyphenols [proanthocyanidin (PA) and quercetin (QC)] with different water solubility were prepared as treatment solutions using ethanol (EtOH) or dimethyl sulfoxide (DMSO) as solvents. 6-um-thick dentin films were microtomed from dentin slabs of third molars. Following demineralization, films or slabs were subject to 60-s treatment (PA or QC) or no treatment (control) with subsequent extended-rinse with original solvent (EtOH or DMSO) or distilled water (DW). Collagen crosslinking interactions were assessed by FTIR. Biostability was assessed through endogenous MMPs activity via confocal laser scanning microscopy, and exogenous collagenase degradation via weight loss, hydroxyproline release and SEM. Finally, direct collagenase inactivation was also evaluated. Data were analyzed by three-way ANOVA and post-hoc tests (α=0.05%).
Distinct effects of two polyphenols and solvents on collagen crosslinking and biostabilization were observed. Higher crosslinking and biostability efficacy occurred with PA than QC (p<0.001) that demonstrated negligible collagen interactions. With DMSO solvent, efficacy results were significantly reduced with both polyphenols (p<0.05). DMSO-rinse further weakened interactions of PA with collagen, diminishing biostability (p<0.05). Low biostability was detected with QC and DW-rinse, suggesting direct enzymatic inhibition due to physical presence in collagen.
Collagen crosslinking interactions and biostability depend on polyphenol chemical characteristics. Treatment-solution solvents may affect interactions between polyphenols and collagen, specifically, DMSO showed detrimental effects on collagen crosslinking and biostability and should be used with caution.
评估不同多酚和溶剂对牙本质胶原交联相互作用的影响，以及对 MMPs 和胶原酶降解的生物稳定性。
用乙醇（EtOH）或二甲基亚砜（DMSO）作为溶剂，制备了两种水溶性不同的多酚[原花青素（PA）和槲皮素（QC）]作为处理液。从第三磨牙的牙本质块上切下 6-μm 厚的牙本质膜。脱矿后，将膜或片用 60s 处理（PA 或 QC）或不处理（对照），然后用原溶剂（EtOH 或 DMSO）或蒸馏水（DW）进行延长冲洗。通过傅里叶变换红外光谱评估胶原交联相互作用。通过共聚焦激光扫描显微镜评估内源性 MMPs 活性，通过失重、羟脯氨酸释放和 SEM 评估外源性胶原酶降解，最后还评估了直接胶原酶失活。采用三因素方差分析和事后检验（α=0.05%）对数据进行分析。
观察到两种多酚和溶剂对胶原交联和生物稳定性的影响明显不同。PA 比 QC 具有更高的交联和生物稳定性效果（p<0.001），而 QC 对胶原的相互作用可以忽略不计。用 DMSO 溶剂，两种多酚的效果都显著降低（p<0.05）。DMSO 冲洗进一步削弱了 PA 与胶原的相互作用，降低了生物稳定性（p<0.05）。QC 和 DW 冲洗的生物稳定性较低，表明由于物理存在于胶原中，直接抑制了酶的活性。
胶原交联相互作用和生物稳定性取决于多酚的化学特性。处理液溶剂可能会影响多酚与胶原之间的相互作用，特别是 DMSO 对胶原交联和生物稳定性有不利影响，应谨慎使用。
