Hansen C E, Meins F
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2492-5. doi: 10.1073/pnas.83.8.2492.
During tumor inception in crown gall disease, a portion of the tumor-inducing (Ti) plasmid, the transferred DNA (T-DNA), is integrated into the genome of the plant cell. Autonomous growth of the transformants requires expression of genes in the tmr and tms regions of the T-DNA, which code for enzymes concerned with biosynthesis of the plant growth hormones cytokinin and auxin, respectively. We show that a mutation of the Habituated leaf gene, Hl, of tobacco can compensate for a defective tmr locus in expression of the tumor phenotype. This provides evidence that a specific host-cell gene has an oncogenic function similar to tmr in the T-DNA.
在冠瘿病肿瘤起始过程中，肿瘤诱导（Ti）质粒的一部分，即转移DNA（T-DNA），会整合到植物细胞的基因组中。转化体的自主生长需要T-DNA的tmr和tms区域中的基因表达，这两个区域分别编码与植物生长激素细胞分裂素和生长素生物合成相关的酶。我们发现，烟草的习性化叶基因Hl发生突变，可以在肿瘤表型表达中补偿有缺陷的tmr基因座。这提供了证据，表明特定的宿主细胞基因具有与T-DNA中的tmr类似的致癌功能。
