Pianotti R, Lachette S, Dills S
J Dent Res. 1986 Jun;65(6):913-7. doi: 10.1177/00220345860650061101.
Fusobacterium nucleatum is a Gram-negative anaerobic rod-shaped bacterium frequently isolated from human dental plaque. It is capable of the desulfuration of cysteine and methionine, resulting in the formation of sulfide and thiol volatiles, respectively. Intact cells, as well as cell-free extracts produced by French pressure cell lysis of F. nucleatum, hydrolyzed radiolabeled cysteine to produce sulfide, pyruvic acid, and ammonia. The hydrolysis products of radiolabeled methionine were a volatile thiol, ketobutyrate, and ammonia. Both activities were associated with the cytoplasmic component, not the membrane. The desulfuration mechanisms are heat-labile, inhibited by the presence of excess substrate, and rates are dependent upon substrate concentration. These dissimilar pathways by F. nucleatum can account in part for the presence of sulfur-containing volatile products that occur in the mouth.
具核梭杆菌是一种革兰氏阴性厌氧杆状细菌，常从人类牙菌斑中分离出来。它能够使半胱氨酸和蛋氨酸脱硫，分别产生硫化物和硫醇挥发物。具核梭杆菌的完整细胞以及通过法国压力细胞裂解产生的无细胞提取物，能将放射性标记的半胱氨酸水解，生成硫化物、丙酮酸和氨。放射性标记蛋氨酸的水解产物是一种挥发性硫醇、酮丁酸和氨。这两种活性都与细胞质成分有关，而非膜成分。脱硫机制对热不稳定，会受到过量底物的抑制，反应速率取决于底物浓度。具核梭杆菌的这些不同途径可以部分解释口腔中含硫挥发性产物的存在。
