Dong Zaiquan, Shen Xiaoling, Hao Yanni, Li Jin, Li Haoran, Xu Haizheng, Yin Li, Kuang Weihong
Mental Health Center of West China Hospital, Sichuan University, Chengdu, China.
Front Psychiatry. 2021 Sep 13;12:651536. doi: 10.3389/fpsyt.2021.651536. eCollection 2021.
Major depressive disorder (MDD) and general anxiety disorder (GAD) share many common features, leading to numerous challenges in their differential diagnosis. Given the importance of the microbiota-gut-brain axis, we investigated the differences in gut microbiota between representative cases of these two diseases and sought to develop a microbiome-based approach for their differential diagnosis. We enrolled 23 patients with MDD, 21 with GAD, and 10 healthy subjects (healthy crowd, HC) in the present study. We used 16S rRNA gene-sequencing analysis to determine the microbial compositions of the gut microbiome based on Illumina Miseq and according to the standard protocol. GAD showed a significant difference in microbiota richness and diversity as compared with HC. Additionally, Otu24167, Otu19140, and Otu19751 were significantly decreased in MDD relative to HC, and Otu2581 and Otu10585 were significantly increased in GAD relative to MDD. At the genus level, the abundances of and were significantly lower in MDD relative to HC, and the abundances of and _R7_group were significantly lower in GAD than in HC. The abundance of was significantly higher whereas that of was significantly lower in GAD relative to MDD. Moreover, we observed that _R7_group negatively correlated with the factor score (Limited to Hopelessness) and total score of HAMD-24 ( < 0.05), whereas negatively correlated with FT4 ( < 0.05). Furthermore, the GAD group showed significant differences at the genus level for , which negatively correlated with PTC ( < 0.05). This study elucidated a unique gut-microbiome signature associated with MDD and GAD that could facilitate differential diagnosis and targeted therapy.
重度抑郁症（MDD）和广泛性焦虑症（GAD）有许多共同特征，这给它们的鉴别诊断带来了诸多挑战。鉴于微生物群-肠道-脑轴的重要性，我们研究了这两种疾病典型病例的肠道微生物群差异，并试图开发一种基于微生物组的鉴别诊断方法。在本研究中，我们招募了23名MDD患者、21名GAD患者和10名健康受试者（健康人群，HC）。我们使用16S rRNA基因测序分析，根据标准方案，基于Illumina Miseq确定肠道微生物组的微生物组成。与HC相比，GAD在微生物群丰富度和多样性上有显著差异。此外，相对于HC，MDD中的Otu24167、Otu19140和Otu19751显著减少，相对于MDD，GAD中的Otu2581和Otu10585显著增加。在属水平上，相对于HC，MDD中 和 的丰度显著降低，GAD中 和_R7_组的丰度显著低于HC。相对于MDD，GAD中 的丰度显著更高，而 的丰度显著更低。此外，我们观察到_R7_组与因子得分（仅限于绝望感）和HAMD-24总分呈负相关（<0.05），而 与FT4呈负相关（<0.05）。此外，GAD组在属水平上 存在显著差异，其与PTC呈负相关（<0.05）。本研究阐明了与MDD和GAD相关的独特肠道微生物组特征，这有助于鉴别诊断和靶向治疗。
