Zaw Aung Moe, Shangguan Richard, Yao Yuan, Jeong YeJin, Yiu Billy Y S, Chee Adrian J Y, Yu Alfred C H, Yim Evelyn K F
Regenerative Nanomedicine Lab, Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada.
Schlegel Research Institute for Aging, Department of Electrical & Computer Engineering, University of Waterloo, Waterloo, ON, Canada.
Methods Mol Biol. 2022;2375:191-201. doi: 10.1007/978-1-0716-1708-3_16.
Conventional ultrasound with frequency (2-15 MHz) has been a global diagnostic and therapeutic tool in clinical medicine, and high-frequency ultrasound (>30 MHz) has been a powerful investigative device for preclinical studies such as cardiovascular research. In this chapter, we describe the use of conventional ultrasound with a 2.5-10 MHz transducer as an investigative device for the measurement/detection of blood flow in rabbit model. The chapter will describe the procedures for the preparation of sonographer, imaging locations, and the details of the rabbits used as well as detailed imaging steps for the preoperative, immediately after operation, and postoperative follow-up ultrasound for vascular surgery, using a vascular graft implantation as an example. We also provide useful notes to avoid pitfalls for successful imaging. The overall goal of this chapter is to deliver the steps in using low-cost, non-invasive, and highly versatile clinical ultrasound imaging in preclinical small animal testing.
频率为2 - 15兆赫的传统超声一直是临床医学中的全球诊断和治疗工具，而高频超声（>30兆赫）则是心血管研究等临床前研究的强大检测设备。在本章中，我们描述了使用配备2.5 - 10兆赫换能器的传统超声作为测量/检测兔模型血流的检测设备。本章将描述超声检查人员的准备程序、成像位置、所用兔子的详细情况，以及以血管移植植入为例的血管手术术前、术后即刻和术后随访超声的详细成像步骤。我们还提供了有用的注意事项，以避免成功成像的陷阱。本章的总体目标是介绍在临床前小动物测试中使用低成本、非侵入性且用途广泛的临床超声成像的步骤。
