Greenfield Edward A
Cold Spring Harb Protoc. 2021 Oct 1;2021(10):2021/10/pdb.prot100289. doi: 10.1101/pdb.prot100289.
Smaller animals such as rats, mice, hamsters, and guinea pigs are usually poor choices for polyclonal antibody production because only small volumes of serum can be obtained. This problem can be reduced by inducing the formation of ascites in mice, which can provide up to 10 mL of ascites fluid from a single animal. Antibody titers in ascites fluids are almost as high as serum titers.
诸如大鼠、小鼠、仓鼠和豚鼠等较小的动物通常不是产生多克隆抗体的理想选择，因为只能获得少量血清。通过在小鼠中诱导腹水形成可以减少这个问题，从一只动物身上最多可获得10毫升腹水。腹水中的抗体效价几乎与血清效价一样高。
