Danis Nilay, Ozkan Aysegul Ertinmaz, Karatas Fatih, Cakici Cagri, Yigitbasi Turkan, Sevencan Nurhayat Ozkan, Kayhan Burcak
Karabuk University Education and Research Hospital, Gastroenterology, Karabuk, Turkey.
Karabuk University Education and Research Hospital, Department of Internal Madicine, Karabuk, Turkey.
J Med Biochem. 2021 Sep 3;40(4):378-383. doi: 10.5937/jomb0-29349.
Oxidative stress status in different cancer types was investigated before, but not studied in gastric intestinal metaplasia to the best of our knowledge. Purpose of this study is to examine whether there is a difference between oxidative stress status in patients with intestinal metaplasia (IM) compared to individuals without IM, we compared the serum levels of disulfide (SS), total thiol (TT) and native thiol (NT).
This was a prospective, non-randomized casecontrol study including 67 patients with histopathologically confirmed IM and 60 individuals demographically matched in terms of age, gender, BMI, smoking status, and chronic diseases as control group.
The mean NT, TT and NT to TT (NT/TT) ratios were statistically significantly higher in IM group compared to controls ((351.71 ± 81.9 mol/L vs. 271.82 ± 54.13 mol/L, p=0.000), (391.5 ± 92.69 mol/L vs. 308.59 ± 55.53 mol/L, p=0.000) and (0.89 ± 0.6 vs. 0.87 ± 0.29, p=0.022), respectively). The mean SS to TT (SS/TT) ratio was significantly lower in IM group than control group (0.050 ± 0.31 vs. 0.060 ± 0.014, P=0.022). Median SS and mean SS/NT ratio was similar in both groups (16.3 (3.3-78) vs. 18.3 (10-32.7), p=0.271 and 0.055 ± 0.041 vs. 0.070 ± 0.019, p=0.068, respectively). In ROC analysis, cut off value of SS/NT for IM was found 0.062, in regression analysis, SS/NT <0.062 was found as an independently prognostic marker for IM (OR, 2.38; 95%CI: 1.168-4.865, P=0.017).
SS/NT ratio lower than 0.062 was found as an independently prognostic marker for IM. This ratio could help to distinguish which patients should be followed closely for gastric cancer.
此前曾对不同癌症类型的氧化应激状态进行过研究，但据我们所知，尚未对胃肠化生中的氧化应激状态进行研究。本研究的目的是检验肠化生（IM）患者与无IM个体的氧化应激状态是否存在差异，我们比较了血清二硫键（SS）、总硫醇（TT）和天然硫醇（NT）水平。
这是一项前瞻性、非随机病例对照研究，包括67例经组织病理学确诊为IM的患者和60例在年龄、性别、体重指数、吸烟状况和慢性病方面人口统计学匹配的个体作为对照组。
IM组的平均NT、TT和NT与TT（NT/TT）比值在统计学上显著高于对照组（分别为（351.71±81.9μmol/L对271.82±54.13μmol/L，p=0.000），（391.5±92.69μmol/L对308.59±55.53μmol/L，p=0.000）和（0.89±0.06对0.87±0.29，p=0.022））。IM组的平均SS与TT（SS/TT）比值显著低于对照组（0.050±0.31对0.060±0.014，P=0.022）。两组的SS中位数和平均SS/NT比值相似（分别为16.3（3.3 - 78）对18.3（10 - 32.7），p=0.271和0.055±0.041对0.070±0.019，p=0.068）。在ROC分析中，发现IM的SS/NT截断值为0.062，在回归分析中，发现SS/NT<0.062是IM的独立预后标志物（OR，2.38；95%CI：1.168 - 4.865，P=0.017）。
发现SS/NT比值低于0.062是IM的独立预后标志物。该比值有助于区分哪些患者应密切随访以防患胃癌。
