Tsukanov Vladislav Vladimirovich, Smirnova Olga Valentinovna, Kasparov Edward Vilyamovich, Sinyakov Alexander Alexandrovich, Vasyutin Alexander Viktorovich, Tonkikh Julia Leongardovna, Cherepnin Mikhail Alexandrovich
Scientific Research Institute of Medical Problems of the North, Federal Research Centre "Krasnoyarsk Science Centre" of the Siberian Branch of Russian Academy of Science, 660022 Krasnoyarsk, Russia.
Diagnostics (Basel). 2022 May 11;12(5):1203. doi: 10.3390/diagnostics12051203.
Gastric cancer is a global health problem. The pathogenesis of this disease remains unclear. This study included 198 (+) men aged 45 to 60 years old. Group A included 63 practically healthy men, group B included 45 men with severe atrophic body gastritis, group C included 37 men with epithelial gastric cancer stages I-II according to TNM, and group D included 54 men with epithelial gastric cancer stages III-IV according to the TNM scale. The content of malondialdehyde (MDA), diene conjugates (DCs), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GPO) was detected using an enzyme immunoassay (ELISA) or spectrophotometric methods in the blood plasma. The concentrations of MDA and DC were increased in the patients of group B compared with group A, and in patients of groups C and D compared with groups A and B. The ratio of MDA/SOD and MDA/CAT was decreased in the patients in group D compared with the patients in group C, and was significantly higher compared with group A. The ratios of MDA/GPO and MDA/GST increased linearly and were at a maximum in groups C and D. Our work determined that indicators of oxidative stress may be the biochemical substrate, which brings together the various stages of the Correa cascade, and may explain disease progression. The dynamics of changes in the content of SOD and CAT in the plasma in patients with gastric cancer may be a target of future investigations.
胃癌是一个全球性的健康问题。这种疾病的发病机制仍不清楚。本研究纳入了198名年龄在45至60岁之间的男性。A组包括63名实际健康的男性，B组包括45名患有严重萎缩性胃体胃炎的男性，C组包括37名根据TNM分期为I-II期的上皮性胃癌男性，D组包括54名根据TNM分期为III-IV期的上皮性胃癌男性。采用酶免疫分析法（ELISA）或分光光度法检测血浆中丙二醛（MDA）、二烯共轭物（DCs）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽S-转移酶（GST）和谷胱甘肽过氧化物酶（GPO）的含量。与A组相比，B组患者的MDA和DC浓度升高，与A组和B组相比，C组和D组患者的MDA和DC浓度升高。与C组患者相比，D组患者的MDA/SOD和MDA/CAT比值降低，与A组相比显著升高。MDA/GPO和MDA/GST比值呈线性增加，在C组和D组中最高。我们的研究确定氧化应激指标可能是生化底物，它将科雷亚级联反应的各个阶段联系在一起，并可能解释疾病的进展。胃癌患者血浆中SOD和CAT含量的变化动态可能是未来研究的目标。
